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Anti-ICAM1 Anticorps [ARC53633 + ARC54995] (A309666)

Nom du produit
Anti-ICAM1 Antibody [ARC53633 + ARC54995]
Description du produit
Rabbit Oligoclonal [ARC53633 + ARC54995] antibody to ICAM1.
Applications
WB, IHC, ICC/IF
Recommander des dilutions
WB: 1:2,000-1:10,000, IHC: 1:100-1:500, ICC/IF: 1:100-1:500
Reactivité
Human, Mouse, Rat
Immunogène
Recombinant protein of human ICAM-1/CD54.
Hôte
Rabbit
Clonalité
Oligoclonal
Clone
ARC53633 + ARC54995
Isotype
IgG
Conjuguer

Unconjugated

Purification
Affinity purification.
Masse moléculaire
89 kDa / 92 kDa
Forme du produit
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.
Stockage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonymes
Antigen identified by monoclonal, BB 2, BB2, CD 54 , CD54, CD54 antigen , CD_antigen=CD54, Cell surface glycoprotein P3.58 , Human rhinovirus receptor , ICAM 1, ICAM-1, ICAM1_HUMAN, Intercellular adhesion molecule 1 , intercellular adhesion molecule 1 (CD54), human rhinovirus receptor, Major group rhinovirus receptor , MALA 2, MALA2 , MGC6195, MyD 10, MyD10, P3.58 , Surface antigen of activated B cells , Surface antigen of activated B cells, BB2
Contrôles isotype
Anticorps secondaires appropriés
Avertissement
Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.
Western Blot - Anti-ICAM1 Antibody [ARC53633 + ARC54995] (A309666) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 1: Western Blot - Anti-ICAM1 Antibody [ARC53633 + ARC54995] (A309666)
Western blot analysis of Mouse lung, using Anti-ICAM1 Antibody [ARC53633 + ARC54995] (A309666) at 1:10,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.
Western Blot - Anti-ICAM1 Antibody [ARC53633 + ARC54995] (A309666) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 2: Western Blot - Anti-ICAM1 Antibody [ARC53633 + ARC54995] (A309666)
Western blot analysis of various lysates, using Anti-ICAM1 Antibody [ARC53633 + ARC54995] (A309666) at 1:10,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 120s.
Immunohistochemistry - Anti-ICAM1 Antibody [ARC53633 + ARC54995] (A309666) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 3: Immunohistochemistry - Anti-ICAM1 Antibody [ARC53633 + ARC54995] (A309666)
Immunohistochemistry analysis of paraffin-embedded mouse lung using Anti-ICAM1 Antibody [ARC53633 + ARC54995] (A309666) at a dilution of 1:300 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunofluorescence - Anti-ICAM1 Antibody [ARC53633 + ARC54995] (A309666) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 4: Immunofluorescence - Anti-ICAM1 Antibody [ARC53633 + ARC54995] (A309666)
Immunofluorescence analysis of HepG2 using Anti-ICAM1 Antibody [ARC53633 + ARC54995] (A309666) at a dilution of 1:400 (40x lens). DAPI was used to stain the cell nuclei (blue).

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