Anti-IKB alpha (phospho Ser36) Anticorps [ARC1543] (A306544)

Name: Anti-IKB alpha (phospho Ser36) Antibody [ARC1543]
Brand: Antibodies.com
SKU: A306544
Price: 540.0 USD
Availability: InStock

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$540

Anticorps monoclonal [ARC1543] de lapin contre IKB alpha (phospho Ser36) pour WB et IHC.

Qualité 100 % Garantie

Garantie de Correspondance des Prix

Livraison

Frais de livraison

$40

Date de livraison

Livraison sous 5-8 jours ouvrables.

Nous ContacterContact

Téléphone

+1 (314) 370-6046

E-mail

orders@antibodies.com

Garanties

Qualité 100 % Garantie

Garantie de Correspondance des Prix

Spécifications

Nom du produit

Anti-IKB alpha (phospho Ser36) Antibody [ARC1543]

Description du produit

Rabbit monoclonal [ARC1543] antibody to IKB alpha (phospho Ser36).

Applications

WB, IHC

Recommander des dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200

Reactivité

Human, Mouse, Rat

Immunogène

A synthetic phosphorylated peptide around S36 of human I?Ba (P25963).

Séquence

LDSMK

Hôte

Rabbit

Clonalité

Monoclonal

Clone

ARC1543

Isotype

IgG

Conjuguer

Unconjugated

Purification

Affinity purification.

Masse moléculaire

39 kDa

Forme du produit

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Stockage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonymes

I kappa B alpha, I-kappa-B-alpha, IkappaBalpha, IkB-alpha, IKBA, IKBalpha, IKBA_HUMAN, MAD 3, MAD3, Major histocompatibility complex enhancer-binding protein MAD3, NF kappa B inhibitor alpha, NF-kappa-B inhibitor alpha, NFKBI, NFKBIA, Nuclear factor of kappa light chain gene enhancer in B cells, Nuclear factor of kappa light polypeptide gene enhancer in B cells inhibitor alpha

Contrôles isotype

Anticorps secondaires appropriés

Avertissement

Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.

Images (4)

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Figure 1: Western Blot - Anti-IKB alpha (phospho Ser36) Antibody [ARC1543] (A306544)

Western blot analysis of extracts of various cell lines, using Anti-IKB alpha (phospho Ser36) Antibody [ARC1543] (A306544) at 1:1,000 dilution. THP-1 cells were treated by PMA/TPA (80 nM) at 37°C for overnight or treated by LPS (1 µg/ml) at 37°C for 6 hours. Both NIH/3T3 cells and C6 cells were treated by TNF-alpha (20 ng/ml) at 37°C for 30 minutes. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% BSA. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 3min.

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Figure 2: Immunohistochemistry - Anti-IKB alpha (phospho Ser36) Antibody [ARC1543] (A306544)

Immunohistochemistry analysis of paraffin-embedded human colon tissue using Anti-IKB alpha (phospho Ser36) Antibody [ARC1543] (A306544) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Figure 3: Immunohistochemistry - Anti-IKB alpha (phospho Ser36) Antibody [ARC1543] (A306544)

Immunohistochemistry analysis of paraffin-embedded mouse liver using Anti-IKB alpha (phospho Ser36) Antibody [ARC1543] (A306544) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-IKB alpha (phospho Ser36) Antibody [ARC1543] (A306544)

Immunohistochemistry analysis of paraffin-embedded rat ovary using Anti-IKB alpha (phospho Ser36) Antibody [ARC1543] (A306544) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.