Figure 1: Western Blot - Anti-IL-10 Antibody [ARC0650] (A307426)
Western blot analysis of extracts of various cell lines, using Anti-IL-10 Antibody [ARC0650] (A307426) at 1:1,000 dilution. THP-1 cells were treated by LPS and Brefeldin A for different times of stimulation. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.
Figure 2: Western Blot - Anti-IL-10 Antibody [ARC0650] (A307426)
Western blot analysis of extracts of Rat thymus, using Anti-IL-10 Antibody [ARC0650] (A307426) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.
Immunofluorescence analysis of Jurkat cells using Anti-IL-10 Antibody [ARC0650] (A307426) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
Immunofluorescence analysis of rat thymus using Anti-IL-10 Antibody [ARC0650] (A307426) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
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