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Anti-KDM1A Anticorps (A13332)

Nom du produit
Anti-KDM1A Antibody
Description du produit
Rabbit polyclonal antibody to KDM1A.
Applications
WB, IF, IP
Recommander des dilutions
WB: 1:500-1:2000, IF: 1:50-1:100, IP: 1:50-1:100
Reactivité
Human, Mouse, Rat
Immunogène
Recombinant fusion protein containing a sequence corresponding to amino acids 130-380 of human KDM1A (NP_055828.2).
Séquence
LSEDEYYSEEERNAKAEKEKKLPPPPPQAPPEEENESEPEEPSGVEGAAFQSRLPHDRMTSQEAACFPDIISGPQQTQKVFLFIRNRTLQLWLDNPKIQLTFEATLQQLEAPYNSDTVLVHRVHSYLERHGLINFGIYKRIKPLPTKKTGKVIIIGSGVSGLAAARQLQSFGMDVTLLEARDRVGGRVATFRKGNYVADLGAMVVTGLGGNPMAVVSKQVNMELAKIKQKCPLYEANGQAVPKEKDEMVEQ
Hôte
Rabbit
Clonalité
Polyclonal
Isotype
IgG
Conjuguer

Unconjugated

Purification
Affinity purification.
Masse moléculaire
96 - 117kDa (Observed MW)
Formulation
Supplied in Phosphate Buffered Saline, pH 7.30, with 0.02% Sodium Azide and 50% Glycerol.
Stockage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonymes
Amine oxidase (flavin containing) domain 2, Amine oxidase, flavin containing, 2, AOF2, BHC110, BRAF35 HDAC complex protein BHC110, BRAF35-HDAC complex protein BHC110, BRAF35/HDAC complex, 110-kD subunit, CPRF, EC1, FAD binding protein BRAF35 HDAC complex, 110 kDa subunit, FAD-binding protein BRAF35-HDAC complex, 110 kDa subunit, Flavin-containing amine oxidase domain-containing protein 2, KDM 1, KDM1, KDM1 / LSD1, KDM1A_HUMAN, KIAA0601, LSD 1, LSD1, Lysine (K) specific demethylase 1, Lysine (K) specific demethylase 1A, Lysine demethylase 1A, Lysine specific histone demethylase 1, Lysine specific histone demethylase 1A, Lysine-specific demethylase 1, Lysine-specific demethylase 1A, Lysine-specific histone demethylase 1, Lysine-specific histone demethylase 1A
Contrôles isotype
Anticorps secondaires appropriés
Avertissement
Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.
Western Blot - Anti-KDM1A Antibody (A1156) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 1: Western Blot - Anti-KDM1A Antibody (A13332)
Western blot analysis of extracts of various cell lines, using Anti-KDM1A Antibody (A1156) at 1:1,000 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L) (HRP) (AS014) at 1:10,000 dilution. Lysates / proteins: 25µg per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit (RM00020).
Western Blot - Anti-KDM1A Antibody (A1156) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 2: Western Blot - Anti-KDM1A Antibody (A13332)
Western blot analysis of extracts from normal (control) and KDM1A knockout (KO) HeLa cells, using Anti-KDM1A Antibody (A1156) at 1:500 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L) (HRP) (AS014) at 1:10,000 dilution. Lysates / proteins: 25µg per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
Immunofluorescence - Anti-KDM1A Antibody (A1156) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 3: Immunofluorescence - Anti-KDM1A Antibody (A13332)
Immunofluorescence analysis of A549 cells using Anti-KDM1A Antibody (A1156).
Immunoprecipitation - Anti-KDM1A Antibody (A1156) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 4: Immunoprecipitation - Anti-KDM1A Antibody (A13332)
Immunoprecipitation analysis of 200µg extracts of HeLa cells using 3µg Anti-KDM1A Antibody (A1156). Western blot was performed from the immunoprecipitate using Anti-KDM1A Antibody (A1156) at a dilition of 1:500.
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