This BAFF enzyme linked immunosorbent assay (ELISA) applies a technique called a quantitative sandwich immunoassay. The microtiter plate has been pre-coated with an Anti-BAFF Antibody. Standards or samples are then added to the microtire plate wells and BAFF, if present, will bind to the antibody pre-coated on the wells. In order to quantify the amount of BAFF present in the sample, Anti-BAFF Antibody (Biotin) is added to each well to "sandwich" the BAFF immobilised on the plate. The microtiter plate then undergoes incubation, followed by thorough washing of the wells to remove all unbound components. Next, Avidin-Biotin-Peroxidase Complex is added to each well for a short incubation period, followed by thorough washing of the wells to remove all unbound conjugates. Next, a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain BAFF will exhibit a change in colour. The enzyme-substrate reaction is terminated by the addition of a stop solution and the colour change is measured spectrophotometrically at a wavelength of 450nm ± 2nm.

Plate-forme

Microplate

Méthode de détection

Colorimetric

Type d'échantillon

Serum, plasma, body fluids, tissue lysates, and cell culture supernatants.

Sensibilité

< 10 pg/ml

Gamme de detection

46.9-3000 pg/ml

Reactivité

Mouse

Precision

Intra-assay CV: < 8%, Inter-assay CV: < 10%

Stockage

Store at 2-8°C for 2 months or at -20°C for 4 months.

Synonymes

ApoL related ligand TALL 1, B cell Activating Factor, B lymphocyte stimulator, B-cell-activating factor, BLyS, CD 257, CD257, CD257 antigen, Delta BAFF, Dendritic cell derived TNF like molecule, Dendritic cell-derived TNF-like molecule, DTL, DTL precursor, PRO738, soluble form, TALL 1, TALL-1, TALL1, THANK, TN13B_HUMAN, TNF and APOL related leukocyte expressed ligand 1, TNF homolog that activates apoptosis, TNF homolog that activates apoptosis NKFB and JNK., TNF- and APOL-related leukocyte expressed ligand 1, TNFSF13B, TNFSF20, TNLG7A, Tumor necrosis factor (ligand) superfamily member 13b, Tumor necrosis factor ligand 7A, Tumor necrosis factor ligand superfamily member 13b, Tumor necrosis factor ligand superfamily member 20, Tumor necrosis factor like protein ZTNF4, Tumor necrosis factor superfamily member 13B, UNQ401, ZTNF4

Avertissement

Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.