This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to the index has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled the index and unlabeled the index (Standards or samples) with the pre-coated antibody specific to the index. After incubation the unbound conjugate is washed off. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of the index in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of the index in the sample.
Plate-forme
Microplate
Méthode de détection
Colorimetric
Type d'échantillon
Serum, plasma and other biological fluids.
Sensibilité
8.83 pg/ml
Gamme de detection
31.25 - 2000 pg/ml
Durée du test
1 - 4.5 Hours
Reactivité
Mouse
Precision
Intra-assay CV: < 8%, Inter-assay CV: < 10%
Synonymes
ADH, Antidiuretic hormone, Arginine vasopressin neurophysin II, ARVP, AVP, AVP NPII, Vasopressin, Vasopressin neurophysin II copeptin, VP
Avertissement
Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.
Produits alternatifs à Souris Copeptin Kit Elisa (A5483)
