Mouse Dkk-1 ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of mouse Dkk-1 in the samples. A monoclonal antibody specific for mouse Dkk-1 has been immobilized onto microwells. Standard or samples are pipetted into the wells, followed by the addition of biotin-linked monoclonal antibody specific for Dkk-1, and Dkk-1 present is bound by the immobilized antibody and detect antibody following the first incubation. After removal of any unbound substances, streptavidin-HRP is added for a second incubation. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of Dkk-1 bound by the immobilized antibody. The color development is stopped by addition of acid and the optical density value is measured by microplate reader.

Plate-forme

Microplate

Méthode de détection

Colorimetric

Type d'échantillon

Cell culture supernatant, serum and plasma.

Sensibilité

3.14 pg/ml

Gamme de detection

93.75 - 6000 pg/ml

Récupération

100% (Average Recovery from Serum Samples).

Spécificité

This kit can assay both natural and recombinant mouse Dkk-1.

Reactivité

Mouse

Réactivité croisée

This assay has shown no significant cross-reactivity.

Precision

Intra-assay CV: < 8%, Inter-assay CV: < 10%

Stockage

Store at 2 - 8°C.

Synonymes

Dickkopf 1, Dickkopf 1 homolog, Dickkopf 1 like, Dickkopf homolog 1 , Dickkopf like protein 1, Dickkopf related protein 1, Dickkopf WNT signaling pathway inhibitor 1, Dickkopf-1, Dickkopf-related protein 1, DKK 1, DKK1, DKK1_HUMAN, hDkk 1 , hDkk-1, hDkk1 , mDkk1, SK

Avertissement

Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.