Souris E Cadherin Kit Elisa (A549)

Mouse E Cadherin ELISA Kit

Sandwich (quantitative)

This E Cadherin enzyme linked immunosorbent assay (ELISA) applies a technique called a quantitative sandwich immunoassay. The microtiter plate has been pre-coated with an Anti-E Cadherin Antibody. Standards or samples are then added to the microtire plate wells and E Cadherin, if present, will bind to the antibody pre-coated on the wells. In order to quantify the amount of E Cadherin present in the sample, Anti-E Cadherin Antibody (Biotin) is added to each well to "sandwich" the E Cadherin immobilised on the plate. The microtiter plate then undergoes incubation, followed by thorough washing of the wells to remove all unbound components. Next, Avidin-Biotin-Peroxidase Complex is added to each well for a short incubation period, followed by thorough washing of the wells to remove all unbound conjugates. Next, a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain E Cadherin will exhibit a change in colour. The enzyme-substrate reaction is terminated by the addition of a stop solution and the colour change is measured spectrophotometrically at a wavelength of 450nm ± 2nm.

Microplate

Serum, plasma, body fluids, tissue lysates, and cell culture supernatants.

< 15 pg/ml

156-10,000 pg/ml

Mouse

Intra-assay CV: < 8%, Inter-assay CV: < 10%

Store at 2-8°C for 4 months or at -20°C for 8 months.

Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.