Mouse IL-12 p70 ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of mouse IL-12 p70 in the samples. A monoclonal antibody specific for mouse IL-12 p70 has been immobilized onto microwells. Standard or samples are pipetted into the wells, followed by the addition of biotin-linked monoclonal antibody specific for IL-12 p70, and IL-12 p70 present is bound by the immobilized antibody and detect antibody following the first incubation. After removal of any unbound substances, streptavidin-HRP is added for a second incubation. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of IL-12 p70 bound by the immobilized antibody. The color development is stopped by addition of acid and the optical density value is measured by microplate reader.
Plate-forme
Microplate
Méthode de détection
Colorimetric
Type d'échantillon
Cell culture supernatant, serum and plasma.
Sensibilité
3.58 pg/ml
Gamme de detection
31.25 - 2000 pg/ml
Récupération
99% (Average Recovery from Serum Samples).
Spécificité
This kit can assay both natural and recombinant mouse IL-12 p70.
Reactivité
Mouse
Réactivité croisée
This assay has shown no significant cross-reactivity.
Precision
Intra-assay CV: < 8%, Inter-assay CV: < 10%
Stockage
Store at 2 - 8°C.
Synonymes
CLMF p35, CLMF p40, Cytotoxic lymphocyte maturation factor 35 kDa subunit , Cytotoxic lymphocyte maturation factor 40 kDa subunit, IL 12A, IL 12B, NK cell stimulatory factor chain 1, NK cell stimulatory factor chain 2
Avertissement
Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.
