Anti-MSH2 Anticorps [ARC55799] (A306655)

Name: Anti-MSH2 Antibody [ARC55799]
Brand: Antibodies.com
SKU: A306655

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Ce produit est discontinué

Anti-MSH2 Anticorps [ARC55799] (A306655) a été abandonné et n'est plus disponible.

Voir toutes Anti-MSH2 Anticorps.

Spécifications

Nom du produit

Anti-MSH2 Antibody [ARC55799]

Description du produit

Rabbit monoclonal [ARC55799] antibody to MSH2.

Applications

WB, IHC

Recommander des dilutions

WB: 1:2,000-1:20,000, IHC: 1:50-1:200

Reactivité

Human, Mouse, Rat

Immunogène

Recombinant fusion protein containing a sequence corresponding to amino acids 330-583 of human MSH2 (NP_000242.1).

Séquence

LNKCKTPQGQRLVNQWIKQPLMDKNRIEERLNLVEAFVEDAELRQTLQEDLLRRFPDLNRLAKKFQRQAANLQDCYRLYQGINQLPNVIQALEKHEGKHQKLLLAVFVTPLTDLRSDFSKFQEMIETTLDMDQVENHEFLVKPSFDPNLSELREIMNDLEKKMQSTLISAARDLGLDPGKQIKLDSSAQFGYYFRVTCKEEKVLRNNKNFSTVDIQKNGVKFTNSKLTSLNEEYTKNKTEYEEAQDAIVKEIVN

Hôte

Rabbit

Clonalité

Monoclonal

Clone

ARC55799

Isotype

IgG

Conjuguer

Unconjugated

Purification

Affinity purification.

Masse moléculaire

105 kDa

Forme du produit

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.

Stockage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonymes

BAT26, COCA 1, COCA1, DNA mismatch repair protein Msh2, FCC 1, FCC1, hMSH2, HNPCC, HNPCC 1, HNPCC1, LCFS2, MSH 2, MSH2_HUMAN, MutS homolog 2, MutS homolog 2 colon cancer nonpolyposis type 1, MutS protein homolog 2

Contrôles isotype

Anticorps secondaires appropriés

Avertissement

Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.

Images (5)

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Figure 1: Western Blot - Anti-MSH2 Antibody [ARC55799] (A306655)

Western blot analysis of extracts from wild type(WT) and MSH2 knockout (KO) HeLa cells, using Anti-MSH2 Antibody [ARC55799] (A306655) at 1:20,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 60s.

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Figure 2: Western Blot - Anti-MSH2 Antibody [ARC55799] (A306655)

Western blot analysis of various lysates, using Anti-MSH2 Antibody [ARC55799] (A306655) at 1:20,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 60s.

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Figure 3: Immunohistochemistry - Anti-MSH2 Antibody [ARC55799] (A306655)

Immunohistochemistry analysis of paraffin-embedded human appendix tissue using Anti-MSH2 Antibody [ARC55799] (A306655) at a dilution of 1:100(40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-MSH2 Antibody [ARC55799] (A306655)

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Anti-MSH2 Antibody [ARC55799] (A306655) at a dilution of 1:100(40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.