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Anti-MUC2 Anticorps (A91628)

Nom du produit
Anti-MUC2 Antibody
Description du produit
Rabbit polyclonal antibody to MUC2.
Applications
WB, IHC, ICC/IF
Recommander des dilutions
WB: 1:500-1:1,000, IHC: 1:100-1:500, ICC/IF: 1:50-1:200
Reactivité
Human, Mouse, Rat
Immunogène
Recombinant fusion protein containing a sequence corresponding to amino acids 21-200 of human MUC2 (NP_002448.4).
Séquence
SELQTEGRTRNHGHNVCSTWGNFHYKTFDGDVFRFPGLCDYNFASDCRGSYKEFAVHLKRGPGQAEAPAGVESILLTIKDDTIYLTRHLAVLNGAVVSTPHYSPGLLIEKSDAYTKVYSRAGLTLMWNREDALMLELDTKFRNHTCGLCGDYNGLQSYSEFLSDGVLFSPLEFGNMQKIN
Hôte
Rabbit
Clonalité
Polyclonal
Isotype
IgG
Conjuguer

Unconjugated

Purification
Affinity purification.
Masse moléculaire
110 kDa / 130 kDa
Forme du produit
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.
Stockage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonymes
Intestinal mucin 2, Intestinal mucin-2, MLP, MUC 2, MUC-2, MUC2_HUMAN, Mucin 2, Mucin 2 intestinal, Mucin 2 intestinal/tracheal, Mucin 2 oligomeric mucus/gel forming, Mucin 2 precursor, Mucin 2 tracheal, Mucin like protein, Mucin-2, Mucin2, SMUC
Contrôles isotype
Anticorps secondaires appropriés
Avertissement
Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.
Western Blot - Anti-MUC2 Antibody (A91628) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 1: Western Blot - Anti-MUC2 Antibody (A91628)
Western blot analysis of extracts of HT-29 cells, using Anti-MUC2 Antibody (A91628) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 180s.
Western Blot - Anti-MUC2 Antibody (A91628) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 2: Western Blot - Anti-MUC2 Antibody (A91628)
Western blot analysis of extracts of various cell lines, using Anti-MUC2 Antibody (A91628) at 1:500 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 180s.
Immunohistochemistry - Anti-MUC2 Antibody (A91628) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 3: Immunohistochemistry - Anti-MUC2 Antibody (A91628)
Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Anti-MUC2 Antibody (A91628) at a dilution of 1:300 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry - Anti-MUC2 Antibody (A91628) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 4: Immunohistochemistry - Anti-MUC2 Antibody (A91628)
Immunohistochemistry analysis of paraffin-embedded human colon tissue using Anti-MUC2 Antibody (A91628) at a dilution of 1:300 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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