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Anti-Myelin Basic Protéine Anticorps (A13581)

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Anti-Myelin Basic Protéine Anticorps [7D2]
Anticorps monoclonal [7D2] de souris contre Myelin Basic Protein pour WB, ICC/IF et IHC.
Nom du produit
Anti-Myelin Basic Protein Antibody
Description du produit
Rabbit polyclonal antibody to Myelin Basic Protein.
Applications
WB, IHC, ICC/IF
Recommander des dilutions
WB: 1:500-1:1,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200
Reactivité
Human, Mouse, Rat
Immunogène
Recombinant fusion protein containing a sequence corresponding to amino acids 80-165 of human Myelin Basic Protein (NP_001020272.1).
Séquence
AHPADPGSRPHLIRLFSRDAPGREDNTFKDRPSESDELQTIQEDSAATSESLDVMASQKRPSQRHGSKYLATASTMDHARHGFLPR
Hôte
Rabbit
Clonalité
Polyclonal
Isotype
IgG
Conjuguer

Unconjugated

Purification
Affinity purification.
Masse moléculaire
12 - 18 kDa
Forme du produit
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.
Stockage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonymes
GDB, Golli MBP, Golli MBP; myelin basic protein, Hemopoietic MBP, HMBPR, HUGO, MBP, MBP_CAVPO, MBP_HUMAN, MGC99675, MLD, Myelin A1 protein, Myelin A1 Protein, basic, Myelin Deficient, Myelin membrane encephalitogenic protein, OTTHUMP00000163776, OTTHUMP00000174387, OTTHUMP00000174388, SHI, Shiverer, SP
Contrôles isotype
Anticorps secondaires appropriés
Avertissement
Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.
Western Blot - Anti-Myelin Basic Protein Antibody (A13581) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 1: Western Blot - Anti-Myelin Basic Protein Antibody (A13581)
Western blot analysis of extracts of various cell lines, using Anti-Myelin Basic Protein Antibody (A13581) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.
Immunohistochemistry - Anti-Myelin Basic Protein Antibody (A13581) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 2: Immunohistochemistry - Anti-Myelin Basic Protein Antibody (A13581)
Immunohistochemistry analysis of paraffin-embedded human brain tissue using Anti-Myelin Basic Protein Antibody (A13581) at a dilution of 1:20 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry - Anti-Myelin Basic Protein Antibody (A13581) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 3: Immunohistochemistry - Anti-Myelin Basic Protein Antibody (A13581)
Immunohistochemistry analysis of paraffin-embedded mouse brain using Anti-Myelin Basic Protein Antibody (A13581) at a dilution of 1:20 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry - Anti-Myelin Basic Protein Antibody (A13581) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 4: Immunohistochemistry - Anti-Myelin Basic Protein Antibody (A13581)
Immunohistochemistry analysis of paraffin-embedded rat brain using Anti-Myelin Basic Protein Antibody (A13581) at a dilution of 1:20 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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