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Anti-N Cadherin Anticorps [ARC0371] (A305345)

Western Blot - Anti-N Cadherin Antibody [ARC0371] (A305345) - Antibodies.com Western Blot - Anti-N Cadherin Antibody [ARC0371] (A305345) - Antibodies.com Immunohistochemistry - Anti-N Cadherin Antibody [ARC0371] (A305345) - Antibodies.com Immunohistochemistry - Anti-N Cadherin Antibody [ARC0371] (A305345) - Antibodies.com Immunofluorescence - Anti-N Cadherin Antibody [ARC0371] (A305345) - Antibodies.com
Anticorps Validé par Knockout
$470
Product Datasheet

Anticorps monoclonal [ARC0371] de lapin contre N Cadherin pour WB, IHC et ICC/IF.

Qualité 100 % Garantie
Garantie de Correspond­ance des Prix

Frais de livraison
$40
Date de livraison
Livraison sous 5-8 jours ouvrables.

Téléphone
+1 (314) 370-6046
Lun - Ven, 8h - 16h AST
E-mail
orders@antibodies.com

Nom du produit
Anti-N Cadherin Antibody [ARC0371]
Description du produit
Rabbit monoclonal [ARC0371] antibody to N Cadherin.
Applications
WB, IHC, ICC/IF
Recommander des dilutions
WB: 1:500-1:2,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200
Reactivité
Human, Mouse, Rat
Immunogène
A synthetic peptide corresponding to a sequence within amino acids 100-200 of human N Cadherin (P19022).
Séquence
AKFLIYAQDKETQEKWQVAVKLSLKPTLTEESVKESAEVEEIVFPRQFSKHSGHLQRQKRDWVIPPINLPENSRGPFPQELVRIRSDRDKNLSLRYSVTGP
Hôte
Rabbit
Clonalité
Monoclonal
Clone
ARC0371
Isotype
IgG
Conjuguer

Unconjugated

Purification
Affinity purification.
Masse moléculaire
140 kDa
Forme du produit
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.
Stockage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonymes
CADH2_HUMAN, Cadherin 2, Cadherin 2 N cadherin neuronal, Cadherin 2 type 1, Cadherin 2 type 1 N cadherin neuronal, Cadherin 2, type 1, N-cadherin (neuronal), Cadherin-2, Cadherin2, Calcium dependent adhesion protein neuronal, CD325, CD325 antigen, CDH2, CDHN, CDw325, CDw325 antigen, N cadherin 1, N-cadherin, NCAD, Neural cadherin, OTTHUMP00000066304, OTTHUMP00000067378
Contrôles isotype
Anticorps secondaires appropriés
Avertissement
Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.
Western Blot - Anti-N Cadherin Antibody [ARC0371] (A305345) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 1: Western Blot - Anti-N Cadherin Antibody [ARC0371] (A305345)
Western blot analysis of extracts from wild type (WT) and N-Cadherin knockout (KO) HeLa cells, using Anti-N Cadherin Antibody [ARC0371] (A305345) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1min.
Western Blot - Anti-N Cadherin Antibody [ARC0371] (A305345) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 2: Western Blot - Anti-N Cadherin Antibody [ARC0371] (A305345)
Western blot analysis of extracts of various cell lines, using Anti-N Cadherin Antibody [ARC0371] (A305345) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1min.
Immunohistochemistry - Anti-N Cadherin Antibody [ARC0371] (A305345) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 3: Immunohistochemistry - Anti-N Cadherin Antibody [ARC0371] (A305345)
Immunohistochemistry analysis of paraffin-embedded mouse heart using Anti-N Cadherin Antibody [ARC0371] (A305345) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry - Anti-N Cadherin Antibody [ARC0371] (A305345) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 4: Immunohistochemistry - Anti-N Cadherin Antibody [ARC0371] (A305345)
Immunohistochemistry analysis of paraffin-embedded rat heart using Anti-N Cadherin Antibody [ARC0371] (A305345) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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