Anti-NADPH oxidase 4 Anticorps (A81032)

Western blot analysis of extracts of 293T cells, using Anti-NADPH oxidase 4 Antibody (A81032) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.

Western blot analysis of extracts from normal (control) and NADPH oxidase 4 (NOX4) Rabbit polyclonal antibody knockout (KO) HeLa cells, using Anti-NADPH oxidase 4 Antibody (A81032) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 60s.

Immunohistochemistry analysis of paraffin-embedded human kidney using Anti-NADPH oxidase 4 Antibody (A81032) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Immunohistochemistry analysis of paraffin-embedded mouse colon using Anti-NADPH oxidase 4 Antibody (A81032) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.