Anti-NF-kB p65 Anticorps (A309621)

Name: Anti-NF-kB p65 Antibody
Brand: Antibodies.com
SKU: A309621

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Ce produit est discontinué

Anti-NF-kB p65 Anticorps (A309621) a été abandonné et n'est plus disponible.

Voir toutes Anti-NF-kB p65 Anticorps.

Spécifications

Nom du produit

Anti-NF-kB p65 Antibody

Description du produit

Rabbit polyclonal antibody to NF-kB p65.

Applications

WB, IHC, ICC/IF

Recommander des dilutions

WB: 1:1,000-1:5,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivité

Human, Mouse, Rat

Immunogène

Recombinant protein of human NF-kB p65/RelA.

Hôte

Rabbit

Clonalité

Polyclonal

Isotype

IgG

Conjuguer

Unconjugated

Purification

Affinity purification.

Masse moléculaire

65 kDa

Forme du produit

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.

Stockage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonymes

Avian reticuloendotheliosis viral (v rel) oncogene homolog A, MGC131774, NF kappa B p65delta3, NFKB3, Nuclear Factor NF Kappa B p65 Subunit, Nuclear factor NF-kappa-B p65 subunit, Nuclear factor of kappa light polypeptide gene enhancer in B cells 3, Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3, OTTHUMP00000233473, OTTHUMP00000233474, OTTHUMP00000233475, OTTHUMP00000233476, OTTHUMP00000233900, p65, p65 NF kappaB, p65 NFkB, relA, TF65_HUMAN, Transcription factor p65, V rel avian reticuloendotheliosis viral oncogene homolog A, v rel avian reticuloendotheliosis viral oncogene homolog A (nuclear factor of kappa light polypeptide gene enhancer in B cells 3 (p65)), v rel reticuloendotheliosis viral oncogene homolog A (avian), V rel reticuloendotheliosis viral oncogene homolog A, nuclear factor of kappa light polypeptide gene enhancer in B cells 3, p65

Contrôles isotype

Anticorps secondaires appropriés

Avertissement

Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.

Images (7)

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Figure 1: Western Blot - Anti-NF-kB p65 Antibody (A309621)

Western blot analysis of various lysates, using Anti-NF-kB p65 Antibody (A309621) at 1:2,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 20s.

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Figure 2: Western Blot - Anti-NF-kB p65 Antibody (A309621)

Western blot analysis of extracts from wild type(WT) and NF-kB p65/RelA knockout (KO) HeLa(KO) cells, using Anti-NF-kB p65 Antibody (A309621) at 1:2,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 20s.

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Figure 3: Immunohistochemistry - Anti-NF-kB p65 Antibody (A309621)

Immunohistochemistry analysis of paraffin-embedded human colon tissue using Anti-NF-kB p65 Antibody (A309621) at a dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-NF-kB p65 Antibody (A309621)

Immunohistochemistry analysis of paraffin-embedded mouse kidney using Anti-NF-kB p65 Antibody (A309621) at a dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.