Anti-NF-kB p65 Anticorps [AMC0222] (A306571)

Name: Anti-NF-kB p65 Antibody [AMC0222]
Brand: Antibodies.com
SKU: A306571

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Ce produit est discontinué

Anti-NF-kB p65 Anticorps [AMC0222] (A306571) a été abandonné et n'est plus disponible.

Voir toutes Anti-NF-kB p65 Anticorps.

Spécifications

Nom du produit

Anti-NF-kB p65 Antibody [AMC0222]

Description du produit

Mouse monoclonal [AMC0222] antibody to NF-kB p65.

Applications

WB, ICC/IF

Recommander des dilutions

WB: 1:500-1:1,000, ICC/IF: 1:50-1:200

Reactivité

Human, Mouse, Rat

Immunogène

A synthetic peptide corresponding to a sequence within amino acids 450-551 of human NF-kB p65/RelA (Q04206).

Séquence

LGALLGNSTDPAVFTDLASVDNSEFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGDEDFSSIADMDFSALLSQISS

Hôte

Mouse

Clonalité

Monoclonal

Clone

AMC0222

Isotype

IgG2b

Chaînes légères

kappa

Conjuguer

Unconjugated

Purification

Affinity purification.

Masse moléculaire

65 kDa

Forme du produit

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Stockage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonymes

Avian reticuloendotheliosis viral (v rel) oncogene homolog A, MGC131774, NF kappa B p65delta3, NFKB3, Nuclear Factor NF Kappa B p65 Subunit, Nuclear factor NF-kappa-B p65 subunit, Nuclear factor of kappa light polypeptide gene enhancer in B cells 3, Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3, OTTHUMP00000233473, OTTHUMP00000233474, OTTHUMP00000233475, OTTHUMP00000233476, OTTHUMP00000233900, p65, p65 NF kappaB, p65 NFkB, relA, TF65_HUMAN, Transcription factor p65, V rel avian reticuloendotheliosis viral oncogene homolog A, v rel avian reticuloendotheliosis viral oncogene homolog A (nuclear factor of kappa light polypeptide gene enhancer in B cells 3 (p65)), v rel reticuloendotheliosis viral oncogene homolog A (avian), V rel reticuloendotheliosis viral oncogene homolog A, nuclear factor of kappa light polypeptide gene enhancer in B cells 3, p65

Contrôles isotype

Anticorps secondaires appropriés

Avertissement

Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.

Images (3)

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Figure 1: Western Blot - Anti-NF-kB p65 Antibody [AMC0222] (A306571)

Western blot analysis of extracts of various cell lines, using Anti-NF-kB p65 Antibody [AMC0222] (A306571) at 1:1,000 dilution. The secondary antibody was HRP Goat Anti-Mouse IgG (H+L) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.

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Figure 2: Immunofluorescence - Anti-NF-kB p65 Antibody [AMC0222] (A306571)

Immunofluorescence analysis of U2OS cells using Anti-NF-kB p65 Antibody [AMC0222] (A306571) at a dilution of 1:100. DAPI was used to stain the cell nuclei (blue).

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Figure 3: Immunofluorescence - Anti-NF-kB p65 Antibody [AMC0222] (A306571)

Immunofluorescence analysis of L929 cells using Anti-NF-kB p65 Antibody [AMC0222] (A306571) at a dilution of 1:100. DAPI was used to stain the cell nuclei (blue).