Anti-NF-M Anticorps (A85323)

Name: Anti-NF-M Antibody
Brand: Antibodies.com
SKU: A85323
Price: 185.0 USD
Availability: InStock

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$185

Anticorps polyclonal de lapin contre NF-M pour WB, ICC/IF et IHC.

Qualité 100 % Garantie

Garantie de Correspondance des Prix

Livraison

Frais de livraison

$40

Date de livraison

Livraison sous 4-6 jours ouvrables.

Nous ContacterContact

Téléphone

+1 (314) 370-6046

E-mail

orders@antibodies.com

Garanties

Qualité 100 % Garantie

Garantie de Correspondance des Prix

Spécifications

Nom du produit

Anti-NF-M Antibody

Description du produit

Rabbit polyclonal antibody to NF-M.

Applications

WB, ICC/IF, IHC

Recommander des dilutions

WB: 1:1,000-1:5,000, ICC/IF: 1:1,000-1:2,500, IHC: 1:1,000-1:2,500

Reactivité

Human, Horse, Bovine, Porcine, Chicken, Rat, Mouse

Immunogène

Recombinant fusion protein corresponding to the extreme C-terminal segment, amino acids 549-845, of rat NF-M.

Hôte

Rabbit

Clonalité

Polyclonal

Isotype

IgG

Conjuguer

Unconjugated

Masse moléculaire

145-160 kDa

Pureté

Whole antiserum.

Forme du produit

Liquid

Formulation

Supplied as an aliquot of serum with 5mM Sodium Azide.

Stockage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonymes

150kDa medium, 160 kDa neurofilament protein, 2 kDa neurofilament protein, 200 kDa neurofilament protein, 68 kDa neurofilament protein, AI847934, CMT1F, CMT2CC, CMT2E, FLJ53642, KIAA0845, light molecular weight neurofilament protein, Micro glutamic acid-rich protein, mKIAA0845, Nef3, NEFH, NEFL, NEFM, Neurofilament, Neurofilament 3, Neurofilament heavy polypeptide, Neurofilament heavy polypeptide 200kDa, Neurofilament light polypeptide, Neurofilament light polypeptide 68kDa, Neurofilament medium polypeptide, Neurofilament medium polypeptide 150kDa, Neurofilament protein heavy polypeptide, Neurofilament protein light chain, Neurofilament protein light polypeptide, Neurofilament protein medium polypeptide, neurofilament subunit NF-L, Neurofilament triplet H protein, Neurofilament triplet L protein, Neurofilament triplet M protein, neurofilament-3 (150 kD medium), NF-H, NF-L, NF160, NF165, NF200, NF68, NFH, NFL, NFM, NFM_HUMAN, PPP1R110, protein phosphatase 1, regulatory subunit 110

Contrôles isotype

Anticorps secondaires appropriés

Avertissement

Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.

Images (4)

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Figure 1: Immunofluorescence - Anti-NF-M Antibody (A85323)

Immunofluorescent analysis of rat cerebellum section stained with Anti-NF-M Antibody (1:2,000 | red) and Anti-GAP43 Antibody (A85393 | 1:2,000 | green). Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45µM, and free-floating sections were stained with the above antibodies. The Anti-NF-M Antibody strongly labels neuronal processes throughout the cerebellum, while the Anti-GAP43 Antibody stains predominantly synaptic regios in the molecular layer.

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Figure 2: Immunofluorescence - Anti-NF-M Antibody (A85323)

Mixed neuron/glia cultures stained with Anti-NF-M Antibody (red) and Anti-Fox1 Antibody (A85396 | green). The Anti-NF-M Antibody stains axonal, dendritic and perikaryal profiles of neurons cleanly and specifically. The Anti-Fox1 Antibody binds to the nuclei of neurons only. DNA is shown in blue with DAPI.

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Figure 3: Western Blot - Anti-NF-M Antibody (A85323)

Western blot analysis of neuronal tissue lysates using Anti-NF-M Antibody (1:2,000 | green): [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord, [6] pig brain, [7] pig spinal cord. Strong bands at 145kDa correspond to rodent NF-M molecules, while the NF-M of pig and other larger mammals including humans run at about 160kDa.

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Figure 4: Western Blot - Anti-NF-M Antibody (A85323)

Blot of rat spinal cord stained with Anti-NF-M Antibody (1: 1,000). A prominent band running with an apparent SDS-PAGE molecular weight of ~145 kDa corresponds to rodent NF-M.