Anti-Nrf2 Anticorps [ARC0806] (A309426)

Name: Anti-Nrf2 Antibody [ARC0806]
Brand: Antibodies.com
SKU: A309426

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Ce produit est discontinué

Anti-Nrf2 Anticorps [ARC0806] (A309426) a été abandonné et n'est plus disponible.

Voir toutes Anti-Nrf2 Anticorps.

Spécifications

Nom du produit

Anti-Nrf2 Antibody [ARC0806]

Description du produit

Rabbit monoclonal [ARC0806] antibody to Nrf2.

Applications

WB, ICC/IF

Recommander des dilutions

WB: 1:100-1:500, ICC/IF: 1:50-1:200

Reactivité

Human

Immunogène

A synthesized peptide derived from human NRF2.

Hôte

Rabbit

Clonalité

Monoclonal

Clone

ARC0806

Isotype

IgG

Conjuguer

Unconjugated

Purification

Affinity purification.

Masse moléculaire

97 kDa

Forme du produit

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Stockage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonymes

erythroid derived 2, HEBP1, like 2, NF E2 related factor 2, NF-E2-related factor 2, NF2L2_HUMAN, NFE2 related factor 2, NFE2-related factor 2, Nfe2l2, Nrf 2, Nuclear factor, Nuclear factor (erythroid derived 2) like 2, nuclear factor erythroid 2 like 2, Nuclear factor erythroid 2 related factor 2, Nuclear factor erythroid 2-related factor 2, Nuclear factor erythroid derived 2 like 2

Contrôles isotype

Anticorps secondaires appropriés

Avertissement

Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.

Images (3)

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Figure 1: Western Blot - Anti-Nrf2 Antibody [ARC0806] (A309426)

Western blot analysis of extracts of HeLa cells, using Anti-Nrf2 Antibody [ARC0806] (A309426) at 1:500 dilution. HeLa cells were treated by MG132(50 µM) at 37°C for 90 minutes. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 60s.

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Figure 2: Western Blot - Anti-Nrf2 Antibody [ARC0806] (A309426)

Western blot analysis of extracts from wild type(WT) and NRF2 knockout (KO) HeLa cells, using Anti-Nrf2 Antibody [ARC0806] (A309426) at 1:500 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 60s.

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Figure 3: Immunofluorescence - Anti-Nrf2 Antibody [ARC0806] (A309426)

Immunofluorescence analysis of HepG2 using Anti-Nrf2 Antibody [ARC0806] (A309426) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).