Recombinant Anti-Nucleolin Anticorps [NCL/7014R] (A278625)

Nom du produit
Anti-Nucleolin Antibody [NCL/7014R] - BSA and Azide free
Description du produit
Recombinant rabbit monoclonal [NCL/7014R] antibody to Nucleolin.
Spécificité
This antibody recognizes a protein of ~76kDa, which is identified as Nucleolin (NCL). It is the major nucleolar phosphoprotein of growing eukaryotic cells. NCL is located mainly in dense fibrillar regions of the nucleolus. It is found associated with intranucleolar chromatin and pre-ribosomal particles. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly.This MAb can be used to stain the nucleoli in cell or tissue preparations and can be used as a marker of the nucleoli in subcellular fractions. It produces a speckled pattern in the nuclei of cells of normal and malignant cells and may be used to stain the nucleoli of cells in fixed or frozen tissue sections. It can be used with paraformaldehyde fixed frozen tissue or cell preparations and formalin fixed, paraffin-embedded tissue sections.
Applications
Flow Cytometry, IF, WB, IHC-P
Recommander des dilutions
Flow Cytometry: 1-2 µg/million cells, IF: 1-2 µg/ml, WB: 1-2 µg/ml, IHC-P: 1-2 µg/ml
Reactivité
Human
Réactivité croisée
This antibody does not cross react with Mouse, Feline, Bovine, Canine, or Horse.
Immunogène
Recombinant full-length human NCL protein.
Hôte
Rabbit
Clonalité
Monoclonal
Clone
NCL/7014R
Isotype
IgG
Chaînes légères
kappa
Conjuguer

Unconjugated

Purification
Protein A/G chromatography.
Concentration
1 mg/ml
Masse moléculaire
76 kDa
Forme du produit
Liquid
Formulation
Supplied in 10mM Phosphate Buffered Saline; without Sodium Azide and carrier free.
Stockage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Notes générales
This monoclonal antibody is also available in a different formulation with BSA and Sodium Azide - Anti-Nucleolin Antibody [NCL/7014R] (A278037).
Synonymes
C23, FLJ45706, MS1116, NCL, Nucl, NUCL_HUMAN, Protein C23
Avertissement
Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.

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