Anti-PCK2 Anticorps [ARC1017] (A305761)

Name: Anti-PCK2 Antibody [ARC1017]
Brand: Antibodies.com
SKU: A305761

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Ce produit est discontinué

Anti-PCK2 Anticorps [ARC1017] (A305761) a été abandonné et n'est plus disponible.

Voir toutes Anti-PCK2 Anticorps.

Spécifications

Nom du produit

Anti-PCK2 Antibody [ARC1017]

Description du produit

Rabbit monoclonal [ARC1017] antibody to PCK2.

Applications

WB, ICC/IF

Recommander des dilutions

WB: 1:500-1:1,000, ICC/IF: 1:50-1:200

Reactivité

Human, Mouse, Rat

Immunogène

Recombinant fusion protein containing a sequence corresponding to amino acids 550-640 of human PEPCK/PEPCK/PCK2 (Q16822).

Séquence

ENARVLDWICRRLEGEDSARETPIGLVPKEGALDLSGLRAIDTTQLFSLPKDFWEQEVRDIRSYLTEQVNQDLPKEVLAELEALERRVHKM

Hôte

Rabbit

Clonalité

Monoclonal

Clone

ARC1017

Isotype

IgG

Conjuguer

Unconjugated

Purification

Affinity purification.

Masse moléculaire

71 kDa

Forme du produit

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Stockage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonymes

EC 4.1.1.32, GTP mitochondrial precursor, HGNC:8725, mitochondrial, Mitochondrial phosphoenolpyruvate carboxykinase 2, OTTHUMP00000164700, PCKGM_HUMAN, PE, PEP carboxykinase, PEPCK, PEPCK deficiency mitochondrial, PEPCK M, PEPCK-M, PEPCK2, Phosphoenolpyruvate carboxykinase 2 (mitochondrial), Phosphoenolpyruvate carboxykinase 2 mitochondrial, Phosphoenolpyruvate carboxykinase [GTP], Phosphoenolpyruvate carboxylase, Phosphopyruvate carboxylase

Contrôles isotype

Anticorps secondaires appropriés

Avertissement

Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.

Images (3)

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Figure 1: Western Blot - Anti-PCK2 Antibody [ARC1017] (A305761)

Western blot analysis of extracts of various cell lines, using Anti-PCK2 Antibody [ARC1017] (A305761) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.

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Figure 2: Western Blot - Anti-PCK2 Antibody [ARC1017] (A305761)

Western blot analysis of extracts from wild type(WT) and PEPCK/PCK2 knockout (KO) 293T cells, using Anti-PCK2 Antibody [ARC1017] (A305761) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

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Figure 3: Immunofluorescence - Anti-PCK2 Antibody [ARC1017] (A305761)

Immunofluorescence analysis of HeLa cells using Anti-PCK2 Antibody [ARC1017] (A305761) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).