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Anti-PCNA Anticorps (A359)

Western Blot of PCNA in HeLa cells and Xenopus egg extract. Anti-PCNA Antibody was used at 1:1,000 dilution. Goat Anti-Rabbit IgG Antibody (HRP) was used as a secondary at 1:10,000 dilution. Lane 1: HeLa cell extract (20µg). Lane 2: Xenopus egg extract (20µg). Molecular mass of PCNA is 29 kDa. Western blot of PCNA in the whole cell extracts. Anti-PCNA Antibody was used at 1:1,000 dilution. Goat Anti-Rabbit IgG Antibody (HRP) was used as secondary at 1:10,000 dilution. Lane 1: Full-size recombinant human PCNA protein (5ng). Lane 2: HeLa (human) (20µg). Lane 3: MCF7 (human) (20µg). Lane 4: NIH3T3 (mouse) (20µg). Lane 5: CHO (hamster) (20µg). Lane 6: Xenopus laevis egg (frog) (20µg). Immunofluorescence staining of PCNA in GM0637 cells (human fibroblast ). Cells were fixed with 4% paraformaldehyde, permeabilized with 0.5% Triton and added with Anti-PCNA Antibody at 1:100 dilution. Goat Anti-Rabbit IgG Antibody (Alexa 488) was used as secondary at 1:10,000 dilution. DNA was stained with Heachst. PCNA foci were detected in the nuclei.
$390

Anticorps polyclonal de lapin contre PCNA pour WB, IP, ICC/IF, DB et ELISA.

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Frais de livraison
$40
Date de livraison
Livraison sous 4-6 jours ouvrables.

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Anti-PCNA Anticorps
Anticorps polyclonal de lapin contre PCNA pour WB, IP et ChIP.
Nom du produit
Anti-PCNA Antibody
Description du produit
Rabbit polyclonal antibody to PCNA.
Applications
WB, IP, ICC/IF, DB, ELISA
Recommander des dilutions
WB: 1:1,000-1:2,000, IP: 1:100-1:500, ICC/IF: 1:100-1:1,000, DB: 1:500-1:2,000, ELISA: 1:500-1:2,000
Reactivité
Human, Mouse, Rat, Hamster, Chicken, Xenopus
Immunogène
Recombinant full length human PCNA protein.
Hôte
Rabbit
Clonalité
Polyclonal
Isotype
IgG
Conjuguer

Unconjugated

Purification
Affinity purification.
Concentration
1 mg/ml
Forme du produit
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 50% Glycerol (filter sterilised, without Sodium Azide, and carrier free).
Stockage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonymes
ATLD2, cb16, Cyclin, DNA polymerase delta auxiliary protein, etID36690.10, fa28e03, fb36g03, HGCN8729, MGC8367, Mutagen-sensitive 209 protein, OTTHUMP00000030189, OTTHUMP00000030190, Pcna/cyclin, PCNAR, PCNA_HUMAN, Polymerase delta accessory protein, Proliferating cell nuclear antigen, wu:fa28e03, wu:fb36g03
Contrôles isotype
Anticorps secondaires appropriés
Avertissement
Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.
Western Blot of PCNA in HeLa cells and Xenopus egg extract. Anti-PCNA Antibody was used at 1:1,000 dilution. Goat Anti-Rabbit IgG Antibody (HRP) was used as a secondary at 1:10,000 dilution. Lane 1: HeLa cell extract (20µg). Lane 2: Xenopus egg extract (20µg). Molecular mass of PCNA is 29 kDa.Cliquez pour ouvrir le point de vue élargi
Figure 1: Western Blot - Anti-PCNA Antibody (70-080)
Anti-PCNA Antibody
Western blot of PCNA in the whole cell extracts. Anti-PCNA Antibody was used at 1:1,000 dilution. Goat Anti-Rabbit IgG Antibody (HRP) was used as secondary at 1:10,000 dilution. Lane 1: Full-size recombinant human PCNA protein (5ng). Lane 2: HeLa (human) (20µg). Lane 3: MCF7 (human) (20µg). Lane 4: NIH3T3 (mouse) (20µg). Lane 5: CHO (hamster) (20µg). Lane 6: Xenopus laevis egg (frog) (20µg).Cliquez pour ouvrir le point de vue élargi
Figure 2: Anti-PCNA Antibody (70-080)
Anti-PCNA Antibody
Immunofluorescence staining of PCNA in GM0637 cells (human fibroblast ). Cells were fixed with 4% paraformaldehyde, permeabilized with 0.5% Triton and added with Anti-PCNA Antibody at 1:100 dilution. Goat Anti-Rabbit IgG Antibody (Alexa 488) was used as secondary at 1:10,000 dilution. DNA was stained with Heachst. PCNA foci were detected in the nuclei.Cliquez pour ouvrir le point de vue élargi
Figure 3: Immunofluorescence - Anti-PCNA Antibody (70-080)
Anti-PCNA Antibody

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