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PDLIM1 Cell Based Kit Elisa (A103016)

Nom du produit
PDLIM1 Cell Based ELISA Kit
Type de test
Cell-based (qualitative)
Principe du test
This PDLIM1 Cell Based ELISA Kit allows for the detection of PDLIM1 and the effects that certain stimulation conditions have on PDLIM1 expression in different cell lines. Qualitative determination of PDLIM1 concentration is achieved by an indirect ELISA format. In essence, the PDLIM1 is captured by Anti-PDLIM1 Antibodies which in turn are detected by HRP-conjugated secondary antibodies. Through this binding, the HRP enzyme (conjugated to the secondary antibody) can catalyze a colorimetric reaction upon substrate addition. Due to the qualitative nature of this PDLIM1 Cell Based ELISA Kit, multiple normalization methods are described: 1) Anti-GAPDH Antibody is included to serve as an internal positive control in normalizing the target absorbance values. 2) Following the colorimetric measurement of HRP activity via substrate addition, the Crystal Violet whole-cell staining method is used to determine cell density. After staining, the results can be analyzed by normalizing the absorbance values to cell amounts, by which the plating difference can be adjusted.
Plate-forme
Microplate
Méthode de détection
Colorimetric
Type d'échantillon
Adherent cells and suspension cells.
Gamme de detection
> 5000 Cells
Durée du test
4 hours 30 minutes
Reactivité
Human, Mouse, Rat
Precision
Intra-assay CV: < 8%, Inter-assay CV: < 10%
Stockage
Store at + 4°C. Stable for 6 months.
Synonymes
C terminal LIM domain protein 1, C-terminal LIM domain protein 1, Carboxy terminal LIM domain protein 1, CLIM 1, CLIM1, CLP 36, CLP36, Elfin, Enigma, Enigma homolog, hCLIM 1, hCLIM1, LIM domain protein CLP 36, LIM domain protein CLP-36, LIM domain protein CLP36, PDLI1_HUMAN, PDLIM 1, PDZ and LIM domain 1, PDZ and LIM domain protein 1
Avertissement
Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.
Western Blot - PDLIM1 Cell Based ELISA Kit (CB5763) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 1: Western Blot - PDLIM1 Cell Based ELISA Kit (CB5763)
Western blot analysis of extracts from Jurkat/COLO205/293/HepG2/HuvEc cells, using Anti-PDLIM1 Antibody.
Protocol Diagram - PDLIM1 Cell Based ELISA Kit (CB5763) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 2: PDLIM1 Cell Based ELISA Kit (CB5763)
A diagram showing the protocol for PDLIM1 Cell Based ELISA Kit.
Protocol Summary - PDLIM1 Cell Based ELISA Kit (CB5763) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 3: PDLIM1 Cell Based ELISA Kit (CB5763)
A summary of the protocol for PDLIM1 Cell Based ELISA Kit.
Western Blot - Control Antibody - PDLIM1 Cell Based ELISA Kit (CB5763) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 4: Western Blot - PDLIM1 Cell Based ELISA Kit (CB5763)
The mouse monoclonal antibody to GAPDH is used as a positive control inPDLIM1 Cell Based ELISA Kit. Anti-GAPDH Antibody was tested for specificity by western blot with tissue lysates from human, mouse, and rat.
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