Anti-PFKFB3 Anticorps [ARC50572] (A306539)

Name: Anti-PFKFB3 Antibody [ARC50572]
Brand: Antibodies.com
SKU: A306539

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Ce produit est discontinué

Anti-PFKFB3 Anticorps [ARC50572] (A306539) a été abandonné et n'est plus disponible.

Voir toutes Anti-PFKFB3 Anticorps.

Spécifications

Nom du produit

Anti-PFKFB3 Antibody [ARC50572]

Description du produit

Rabbit monoclonal [ARC50572] antibody to PFKFB3.

Applications

WB, IHC

Recommander des dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:200

Reactivité

Human, Mouse, Rat

Immunogène

A synthetic peptide corresponding to a sequence within amino acids 400-500 of human PFKFB3 (NP_004557.1).

Séquence

LDKSAEEMPYLKCPLHTVLKLTPVAYGCRVESIYLNVESVCTHRERSEDAKKGPNPLMRRNSVTPLASPEPTKKPRINSFEEHVASTSAALPSCLPPEVPT

Hôte

Rabbit

Clonalité

Monoclonal

Clone

ARC50572

Isotype

IgG

Conjuguer

Unconjugated

Purification

Affinity purification.

Masse moléculaire

60 kDa

Forme du produit

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.

Stockage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonymes

6 phosphofructo 2 kinase/ fructose 2,6 bisphosphatase, 6 phosphofructo 2 kinase/fructose 2,6 biphosphatase 3, 6-bisphosphatase, 6-P2ase 3, 6-P2ASE brain/placenta-type isozyme, 6PF 2 K/Fru 2,6 P2ASE brain/placenta type isozyme, 6PF 2-K/Fru 2,6 P2ase 3, 6PF-2-K/Fru-2, F263_HUMAN, fructose 6 phosphate,2 kinase/fructose 2, 6 bisphosphatase, Fructose-2, Inducible 6 phosphofructo 2 kinase/fructose 2,6 bisphosphatase, iPFK 2, iPFK-2, IPFK2, PFK/FBPase 3, PFK2, Renal carcinoma antigen NY REN 56, Renal carcinoma antigen NY-REN-56, uPFK 2

Contrôles isotype

Anticorps secondaires appropriés

Avertissement

Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.

Images (5)

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Figure 1: Western Blot - Anti-PFKFB3 Antibody [ARC50572] (A306539)

Western blot analysis of HeLa, using Anti-PFKFB3 Antibody [ARC50572] (A306539) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.

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Figure 2: Western Blot - Anti-PFKFB3 Antibody [ARC50572] (A306539)

Western blot analysis of Mouse brain, using Anti-PFKFB3 Antibody [ARC50572] (A306539) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 60s.

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Figure 3: Immunohistochemistry - Anti-PFKFB3 Antibody [ARC50572] (A306539)

Immunohistochemistry analysis of paraffin-embedded human breast cancer tissue using Anti-PFKFB3 Antibody [ARC50572] (A306539) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-PFKFB3 Antibody [ARC50572] (A306539)

Immunohistochemistry analysis of paraffin-embedded mouse spinal cord using Anti-PFKFB3 Antibody [ARC50572] (A306539) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.