Anti-PHD3 Anticorps [ARC52452] (A307618)

Name: Anti-PHD3 Antibody [ARC52452]
Brand: Antibodies.com
SKU: A307618

Cliquez pour ouvrir le point de vue élargi

Ce produit est discontinué

Anti-PHD3 Anticorps [ARC52452] (A307618) a été abandonné et n'est plus disponible.

Voir toutes Anti-PHD3 Anticorps.

Spécifications

Nom du produit

Anti-PHD3 Antibody [ARC52452]

Description du produit

Rabbit monoclonal [ARC52452] antibody to PHD3.

Applications

WB, IHC, ICC/IF

Recommander des dilutions

WB: 1:2,000-1:20,000, IHC: 1:500-1:1,000, ICC/IF: 1:50-1:200

Reactivité

Human, Mouse, Rat

Immunogène

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human PHD3 (NP_071356.1).

Séquence

MPLGHIMRLDLEKIALEYIVPCLHEVGFCYLDNFLGEVVGDCVLERVKQLHCTGALRDGQLAGPRAGVSKRHLRGDQITWIGGNEEGCEAISFLLSLIDR

Hôte

Rabbit

Clonalité

Monoclonal

Clone

ARC52452

Isotype

IgG

Conjuguer

Unconjugated

Purification

Affinity purification.

Masse moléculaire

32 kDa

Forme du produit

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.

Stockage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonymes

Egl 9 family hypoxia inducible factor 3, Egl nine homolog 3, Egl nine homolog 3 (C. elegans), Egl nine like protein 3 isoform, EGL9 homolog of C. elegans 3, EGLN3, EGLN3_HUMAN, Factor responsive smooth muscle protein, HIF Prolyl Hydroxylase 3, HIF-PH3, HIF-prolyl hydroxylase 3, HIFP4H3, HIFPH3, HPH-1, HPH-3, Hypoxia-inducible factor prolyl hydroxylase 3, P4H3, Prolyl Hydroxylase Domain Containing Protein 3, Prolyl hydroxylase domain-containing protein 3, SM20

Contrôles isotype

Anticorps secondaires appropriés

Avertissement

Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.

Images (7)

Cliquez pour ouvrir le point de vue élargi

Figure 1: Western Blot - Anti-PHD3 Antibody [ARC52452] (A307618)

Western blot analysis of extracts of various cell lines, using Anti-PHD3 Antibody [ARC52452] (A307618) at 1:20,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 60s.

Cliquez pour ouvrir le point de vue élargi

Figure 2: Western Blot - Anti-PHD3 Antibody [ARC52452] (A307618)

Western blot analysis of extracts of BxPC-3 cells, using Anti-PHD3 Antibody [ARC52452] (A307618) at 1:20,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.

Cliquez pour ouvrir le point de vue élargi

Figure 3: Immunohistochemistry - Anti-PHD3 Antibody [ARC52452] (A307618)

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Anti-PHD3 Antibody [ARC52452] (A307618) at a dilution of 1:1,000 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Cliquez pour ouvrir le point de vue élargi

Figure 4: Immunohistochemistry - Anti-PHD3 Antibody [ARC52452] (A307618)

Immunohistochemistry analysis of paraffin-embedded human esophageal cancer using Anti-PHD3 Antibody [ARC52452] (A307618) at a dilution of 1:1,000 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.