Anti-PSGL-1 Anticorps [ARC55288] (A309636)

Name: Anti-PSGL-1 Antibody [ARC55288]
Brand: Antibodies.com
SKU: A309636

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Ce produit est discontinué

Anti-PSGL-1 Anticorps [ARC55288] (A309636) a été abandonné et n'est plus disponible.

Voir toutes Anti-PSGL-1 Anticorps.

Spécifications

Nom du produit

Anti-PSGL-1 Antibody [ARC55288]

Description du produit

Rabbit monoclonal [ARC55288] antibody to PSGL-1.

Applications

WB, IHC, ICC/IF

Recommander des dilutions

WB: 1:10,000-1:40,000, IHC: 1:500-1:1,000, ICC/IF: 1:500-1:1,000

Reactivité

Human, Mouse, Rat

Immunogène

Recombinant protein of human CD162/PSGL-1.

Hôte

Rabbit

Clonalité

Monoclonal

Clone

ARC55288

Isotype

IgG

Conjuguer

Unconjugated

Purification

Affinity purification.

Masse moléculaire

122 kDa

Forme du produit

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.

Stockage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonymes

CD 162, CD162, CD162 antigen, CLA, Cutaneous lymphocyte associated associated antigen, P selectin glycoprotein ligand 1, P selectin glycoprotein ligand 1 precursor, P-selectin glycoprotein ligand 1, PSGL 1, PSGL1, Selectin P ligand, SELPLG, SELPL_HUMAN

Contrôles isotype

Anticorps secondaires appropriés

Avertissement

Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.

Images (6)

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Figure 1: Western Blot - Anti-PSGL-1 Antibody [ARC55288] (A309636)

Western blot analysis of various lysates, using Anti-PSGL-1 Antibody [ARC55288] (A309636) at 1:40,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

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Figure 2: Western Blot - Anti-PSGL-1 Antibody [ARC55288] (A309636)

Western blot analysis of Mouse spleen, using Anti-PSGL-1 Antibody [ARC55288] (A309636) at 1:40,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

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Figure 3: Immunohistochemistry - Anti-PSGL-1 Antibody [ARC55288] (A309636)

Immunohistochemistry analysis of paraffin-embedded human spleen using Anti-PSGL-1 Antibody [ARC55288] (A309636) at a dilution of 1:1,000 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-PSGL-1 Antibody [ARC55288] (A309636)

Immunohistochemistry analysis of paraffin-embedded human tonsil using Anti-PSGL-1 Antibody [ARC55288] (A309636) at a dilution of 1:1,000 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.