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PTTG Cell Based Kit Elisa (A103287)

Nom du produit
PTTG Cell Based ELISA Kit
Type de test
Cell-based (qualitative)
Principe du test
This PTTG Cell Based ELISA Kit allows for the detection of PTTG and the effects that certain stimulation conditions have on PTTG expression in different cell lines. Qualitative determination of PTTG concentration is achieved by an indirect ELISA format. In essence, the PTTG is captured by Anti-PTTG Antibodies which in turn are detected by HRP-conjugated secondary antibodies. Through this binding, the HRP enzyme (conjugated to the secondary antibody) can catalyze a colorimetric reaction upon substrate addition. Due to the qualitative nature of this PTTG Cell Based ELISA Kit, multiple normalization methods are described: 1) Anti-GAPDH Antibody is included to serve as an internal positive control in normalizing the target absorbance values. 2) Following the colorimetric measurement of HRP activity via substrate addition, the Crystal Violet whole-cell staining method is used to determine cell density. After staining, the results can be analyzed by normalizing the absorbance values to cell amounts, by which the plating difference can be adjusted.
Plate-forme
Microplate
Méthode de détection
Colorimetric
Type d'échantillon
Adherent cells and suspension cells.
Gamme de detection
> 5000 Cells
Durée du test
4 hours 30 minutes
Reactivité
Human, Mouse, Rat
Precision
Intra-assay CV: < 8%, Inter-assay CV: < 10%
Stockage
Store at + 4°C. Stable for 6 months.
Synonymes
AW555095, C87862, Cut2, EAP 1, EAP1, ESP1 associated protein 1, Esp1-associated protein, hPTTG, MGC126883, MGC138276, Pds1, Pituitary tumor transforming 1, Pituitary tumor transforming protein 1, Pituitary tumor-transforming 1, isoform CRA_a, Pituitary tumor-transforming 1, isoform CRA_b, Pituitary tumor-transforming gene 1, Pituitary tumor-transforming gene 1 protein, PTTG 1, PTTG1, PTTG1 protein, PTTG1_HUMAN, Pttg3, Securin, Tumor transforming 1, Tumor transforming protein 1, Tumor-transforming protein 1, TUTR 1, TUTR1
Avertissement
Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.
Western Blot - PTTG Cell Based ELISA Kit (CB5923) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 1: Western Blot - PTTG Cell Based ELISA Kit (CB5923)
Western blot analysis of extracts from RAW264.7 cells, using Anti-PTTG Antibody.
Protocol Diagram - PTTG Cell Based ELISA Kit (CB5923) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 2: PTTG Cell Based ELISA Kit (CB5923)
A diagram showing the protocol for PTTG Cell Based ELISA Kit.
Protocol Summary - PTTG Cell Based ELISA Kit (CB5923) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 3: PTTG Cell Based ELISA Kit (CB5923)
A summary of the protocol for PTTG Cell Based ELISA Kit.
Western Blot - Control Antibody - PTTG Cell Based ELISA Kit (CB5923) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 4: Western Blot - PTTG Cell Based ELISA Kit (CB5923)
The mouse monoclonal antibody to GAPDH is used as a positive control inPTTG Cell Based ELISA Kit. Anti-GAPDH Antibody was tested for specificity by western blot with tissue lysates from human, mouse, and rat.
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