This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to the index has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled the index and unlabeled the index (Standards or samples) with the pre-coated antibody specific to the index. After incubation the unbound conjugate is washed off. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of the index in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of the index in the sample.

Plate-forme

Microplate

Méthode de détection

Colorimetric

Type d'échantillon

Serum, plasma and other biological fluids.

Sensibilité

16.23 pg/ml

Gamme de detection

46.875 - 3000 pg/ml

Durée du test

1 - 4.5 Hours

Reactivité

Rat

Precision

Intra-assay CV: < 8%, Inter-assay CV: < 10%

Synonymes

BRST-2, BRST2, GCDFP 15, GCDFP-15, GCDGP15, gp17, GPIP4, Gross cystic disease fluid protein 15, PIP, PIP_HUMAN, Prolactin inducible protein precursor, Prolactin-induced protein, Prolactin-inducible protein, SABP, Secretory actin binding protein, Secretory actin-binding protein

Avertissement

Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.

Images (1)

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Figure 1: Standard Curve - Rat Prolactin Induced Protein ELISA Kit (DL-PIP-Ra)

Representative Standard Curve using Rat Prolactin Induced Protein ELISA Kit (DL-PIP-Ra)

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