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Recombinant Fel d 1 (1.0101) Protéine (A242920)

Elisa Test - Recombinant Fel d 1 (1.0101) Protein (A242919) - Antibodies.com SDS-PAGE - Recombinant Fel d 1 (1.0101) Protein (A242920) - Antibodies.com Flow Cytometry - Recombinant Fel d 1 (1.0101) Protein (A242920) - Antibodies.com
$525
Product Datasheet
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Garantie de Correspond­ance des Prix

Frais de livraison
$40
Date de livraison
Livraison sous 4-6 jours ouvrables.

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Nom du produit
Recombinant Fel d 1 (1.0101) Protein
Applications
Flow Cytometry, ELISA, WB
Système d'expression
Escherichia coli
Nature
Recombinant
Espèces de protéines
Felis domesticus (Cat)
Séquence
DNA sequence encoding 175 amino acids was fused with a Strep Tag at the N-terminus to form this recombinant protein.
Étiquette
Strep Tag (N-terminus)
MW prédit
19.4 kDa
Conjuguer

Unconjugated

Purification
Ion exchange and affinity chromatography.
Forme du produit
Lyophilized
Concentration
Reconstitution dependent.
Formulation
Lyophilized from 100mM Tris Buffer, pH 8, with 150mM NaCl, and without preservatives or carriers (0.2 µm filter sterilized).
Stockage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C. Reconstituted: Use immediately or aliquot and store at -20°C to -80°C. Avoid freeze/thaw cycles.
Avertissement
Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.
Elisa Test - Recombinant Fel d 1 (1.0101) Protein (A242919) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 1: Recombinant Fel d 1 (1.0101) Protein (A242920)
An ELISA test was designed to prove the bond between the coated target, Recombinant Fel d 1 (1.0101) Protein, and allergen-specific human plasma IgG4 antibodies of a Felis domesticus alergy positive donor. A measurable signal was subsequently generated by the addition of biotin labeled anti-human IgG4 (detection) antibodies, Streptavidin-HRP and substrate solution (TMB). The intensity of the signal is proportional to the amount of coated Recombinant Fel d 1 (1.0101) Protein.
SDS-PAGE - Recombinant Fel d 1 (1.0101) Protein (A242920) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 2: SDS-PAGE - Recombinant Fel d 1 (1.0101) Protein (A242920)
Purity verification: 5 µg of Recombinant Fel d 1 (1.0101) Protein with > 95 % purity checked by Coomassie Brilliant Blue stained SDS-PAGE.
Flow Cytometry - Recombinant Fel d 1 (1.0101) Protein (A242920) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 3: Flow Cytometry - Recombinant Fel d 1 (1.0101) Protein (A242920)
Flow cytometry dot-plot staining pattern of Recombinant Fel d 1 (1.0101) Protein stimulated human peripheral whole blood lymphocytes and basophils of a proven allergic donor stained using Anti-CD63 [MEM-259] (FITC) Antibody and Anti-CD203c [NP4D6] (PE) Antibody.
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