Recombinant Human IL-2 Protein (mutated L100F + R101D + L105V + I106V + I112F) (6xHis Tag)
Applications
Cell Proferation Assay, SDS-PAGE
Système d'expression
HEK293 cells
Nature
Recombinant
Espèces de protéines
Human
Longueur des protéines
Protein fragment.
Acides aminés
Ala21-Thr153 (L100F, R101D, L105V, I106V, I112F)
Étiquette
C-terminal 6xHis Tag
Masse moléculaire
16.5 KDa
Conjuguer
Unconjugated
Pureté
> 95%, by SDS-PAGE under reducing conditions.
Forme du produit
Lyophilized
Concentration
Reconstitution dependent.
Formulation
Lyophilized from a 0.2 µm filtered solution of 4mM HCl.
Stockage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C. Reconstituted: Aliquot and store at -80°C. Product is stable for one year. Avoid freeze/thaw cycles.
Synonymes
Aldesleukin, IL 2, IL2, IL2_HUMAN, Interleukin 2, Interleukin-2, Interleukin2, Involved in regulation of T cell clonal expansion, Lymphokine , OTTHUMP00000164090, POIL2, T cell growth factor, T-cell growth factor, TCGF
Avertissement
Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.
Figure 2: Recombinant Human IL-2 Protein (mutated L100F + R101D + L105V + I106V + I112F) (6xHis Tag) (A317605)
Loaded Human IL-2RB-Fc on Protein A Biosensor, can bind Recombinant Human IL-2 Protein (mutated L100F + R101D + L105V + I106V + I112F) (6×His Tag) (A317605) with an affinity constant of 0.58 nM as determined in BLI assay.
Figure 3: Recombinant Human IL-2 Protein (mutated L100F + R101D + L105V + I106V + I112F) (6xHis Tag) (A317605)
Measurement of Recombinant Human IL-2 Protein (mutated L100F + R101D + L105V + I106V + I112F) (6×His Tag) (A317605) in a cell proliferation assay using CTLL-2 mouse cytotoxic T cells. The ED50 for this effect is 151.39 pg/ml.
Figure 4: Recombinant Human IL-2 Protein (mutated L100F + R101D + L105V + I106V + I112F) (6xHis Tag) (A317605)
Loaded Human IL-2RA-Fc on Protein A Biosensor, can bind Recombinant Human IL-2 Protein (mutated L100F + R101D + L105V + I106V + I112F) (6×His Tag) (A317605) with an affinity constant of 10.4 nM as determined in BLI assay.
