Anti-RNA Polymerase II CTD repeat YSPTSPS (phospho Ser5) Anticorps [CTD 8A7] (A251056)

Anti-RNA Polymerase II CTD repeat YSPTSPS (phospho Ser5) Antibody [CTD 8A7]

Mouse monoclonal [CTD 8A7] antibody to RNA Polymerase II CTD repeat YSPTSPS (phospho Ser5).

RNA polymerase II (Pol II) is an enzyme that is composed of 12 subunits and is responsible for the transcription of protein-coding genes. Transcription initiation requires Pol II-mediated recruitment of transcription machinery to a target promoter, thereby allowing transcription to begin. The largest subunit of Pol II (referred to as RPB1 or RPB205) is a 1,840 amino acid protein that contains one C2H2-type zinc finger and a C-terminal domain comprised of several heptapeptide repeats. Although Pol II function requires the cooperation of all twelve subunits, the largest subunit conveys Pol II catalytic activity and, together with the second largest subunit, forms the active center of the Pol II enzyme. Additionally, the large subunit participates in forming the DNA-binding domain of Pol II, a groove that is necessary for transcription of the DNA template. Without proper function of the large subunit, mRNA synthesis and subsequent transcription elongation cannot occur.

Flow Cytometry, IF, WB, IHC-P

Flow Cytometry: 1-2 µg/million cells, IF: 1-2 µg/ml, WB: 1-2 µg/ml, IHC-P: 1-2 µg/ml

Human, Saccharomyces cerevisiae

Ten repeats of synthetic peptide YSPTSPS using chemically synthesized phospho Ser5.

Mouse

Monoclonal

CTD 8A7

IgM

Unconjugated

200 µg/ml

192-253 kDa

Liquid

Supplied in 10mM Phosphate Buffered Saline with 0.05% BSA and 0.05% Sodium Azide.

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

This monoclonal antibody is also available in a different formulation without BSA and Sodium Azide - Anti-RNA Polymerase II CTD repeat YSPTSPS (phospho Ser5) Antibody [CTD 8A7] - BSA and Azide free (A254333).

DNA directed RNA polymerase II A, DNA-directed RNA polymerase II largest subunit RNA polymerase II 220 kd subunit, DNA-directed RNA polymerase II subunit A, DNA-directed RNA polymerase II subunit RPB1, DNA-directed RNA polymerase III largest subunit, hRPB220, hsRPB1, POLR2, Polr2a, POLRA, Polymerase (RNA) II (DNA directed) polypeptide A, Polymerase (RNA) II (DNA directed) polypeptide A 220kDa, RNA polymerase II subunit B1, RNA-directed RNA polymerase II subunit RPB1, RPB1, RPB1_HUMAN, RPBh1, RpIILS, RPO2, RPOL2