Anti-S100 alpha Anticorps [ARC59163] (A309671)

Name: Anti-S100 alpha Antibody [ARC59163]
Brand: Antibodies.com
SKU: A309671

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Ce produit est discontinué

Anti-S100 alpha Anticorps [ARC59163] (A309671) a été abandonné et n'est plus disponible.

Voir toutes Anti-S100 alpha Anticorps.

Spécifications

Nom du produit

Anti-S100 alpha Antibody [ARC59163]

Description du produit

Rabbit monoclonal [ARC59163] antibody to S100 alpha.

Applications

WB, IHC, ICC/IF

Recommander des dilutions

WB: 1:500-1:1,000, IHC: 1:100-1:500, ICC/IF: 1:50-1:200

Reactivité

Human, Mouse, Rat

Immunogène

Recombinant protein of human S100/S100A1.

Hôte

Rabbit

Clonalité

Monoclonal

Clone

ARC59163

Isotype

IgG

Conjuguer

Unconjugated

Purification

Affinity purification.

Masse moléculaire

12 kDa

Forme du produit

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.

Stockage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonymes

Bpb, NEF, Protein S100-A1, S-100 protein alpha chain, S-100 protein subunit alpha, S100, S100 Alpha Chain, S100 Beta Chain, S100 Calcium Binding Protein A1, S100 Calcium Binding Protein B, S100 Calcium Binding Protein Beta Neural, S100 calcium-binding protein A1, S100 protein alpha polypeptide, S100A, s100a1, S10A1_HUMAN

Contrôles isotype

Anticorps secondaires appropriés

Avertissement

Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.

Images (4)

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Figure 1: Western Blot - Anti-S100 alpha Antibody [ARC59163] (A309671)

Western blot analysis of various lysates, using Anti-S100 alpha Antibody [ARC59163] (A309671) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.

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Figure 2: Immunohistochemistry - Anti-S100 alpha Antibody [ARC59163] (A309671)

Immunohistochemistry analysis of paraffin-embedded mouse heart using Anti-S100 alpha Antibody [ARC59163] (A309671) at a dilution of 1:500 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 3: Immunohistochemistry - Anti-S100 alpha Antibody [ARC59163] (A309671)

Immunohistochemistry analysis of paraffin-embedded rat brain using Anti-S100 alpha Antibody [ARC59163] (A309671) at a dilution of 1:500 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 4: Immunofluorescence - Anti-S100 alpha Antibody [ARC59163] (A309671)

Immunofluorescence analysis of HepG2 cells using Anti-S100 alpha Antibody [ARC59163] (A309671) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).