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Anti-S100A4 Anticorps [ARC0475] (A305282)

Nom du produit
Anti-S100A4 Antibody [ARC0475]
Description du produit
Rabbit monoclonal [ARC0475] antibody to S100A4.
Applications
WB, IHC, ICC/IF
Recommander des dilutions
WB: 1:500-1:2,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200
Reactivité
Human, Mouse, Rat
Immunogène
Recombinant fusion protein containing a sequence corresponding to amino acids 2-101 of human S100A4 (P26447).
Séquence
ACPLEKALDVMVSTFHKYSGKEGDKFKLNKSELKELLTRELPSFLGKRTDEAAFQKLMSNLDSNRDNEVDFQEYCVFLSCIAMMCNEFFEGFPDKQPRKK
Hôte
Rabbit
Clonalité
Monoclonal
Clone
ARC0475
Isotype
IgG
Conjuguer

Unconjugated

Purification
Affinity purification.
Masse moléculaire
12 kDa
Forme du produit
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.
Stockage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonymes
18A2, 42A, calcium Placental protein, Calvasculin, CAPL, Fibroblast specific protein, Fibroblast specific protein 1, Fibroblast specific protein 1 (FSP1), FSP1, Leukemia multidrug resistance associated protein, Malignant transformation suppression 1, Malignant transformation suppression 1 (MTS1), Metastasin, MTS1, OTTHUMP00000015467, OTTHUMP00000015468, P9KA, PEL98, Placental calcium-binding protein, Protein Mts1, Protein S100 A4, Protein S100-A4, S100 calcium binding protein A4, S100 calcium binding protein A4 (calcium protein, calvasculin, metastasin, murine placental homolog), S100 calcium-binding protein A4, S10A4_HUMAN
Contrôles isotype
Anticorps secondaires appropriés
Avertissement
Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.
Western Blot - Anti-S100A4 Antibody [ARC0475] (A305282) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 1: Western Blot - Anti-S100A4 Antibody [ARC0475] (A305282)
Western blot analysis of extracts of various cell lines, using Anti-S100A4 Antibody [ARC0475] (A305282) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 60s.
Immunohistochemistry - Anti-S100A4 Antibody [ARC0475] (A305282) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 2: Immunohistochemistry - Anti-S100A4 Antibody [ARC0475] (A305282)
Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Anti-S100A4 Antibody [ARC0475] (A305282) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry - Anti-S100A4 Antibody [ARC0475] (A305282) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 3: Immunohistochemistry - Anti-S100A4 Antibody [ARC0475] (A305282)
Immunohistochemistry analysis of paraffin-embedded mouse spleen using Anti-S100A4 Antibody [ARC0475] (A305282) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry - Anti-S100A4 Antibody [ARC0475] (A305282) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 4: Immunohistochemistry - Anti-S100A4 Antibody [ARC0475] (A305282)
Immunohistochemistry analysis of paraffin-embedded rat lung using Anti-S100A4 Antibody [ARC0475] (A305282) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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