Anti-SIAH1 Anticorps [ARC59737] (A309470)

Name: Anti-SIAH1 Antibody [ARC59737]
Brand: Antibodies.com
SKU: A309470

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Ce produit est discontinué

Anti-SIAH1 Anticorps [ARC59737] (A309470) a été abandonné et n'est plus disponible.

Voir toutes Anti-SIAH1 Anticorps.

Spécifications

Nom du produit

Anti-SIAH1 Antibody [ARC59737]

Description du produit

Rabbit monoclonal [ARC59737] antibody to SIAH1.

Applications

WB, IHC

Recommander des dilutions

WB: 1:1,000-1:5,000, IHC: 1:50-1:200

Reactivité

Human, Mouse, Rat

Immunogène

Recombinant protein of human SIAH1.

Hôte

Rabbit

Clonalité

Monoclonal

Clone

ARC59737

Isotype

IgG

Conjuguer

Unconjugated

Purification

Affinity purification.

Masse moléculaire

31 kDa / 35 kDa

Forme du produit

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.

Stockage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonymes

E3 ubiquitin-protein ligase SIAH1, FLJ08065, hSIAH1, HUMSIAH, Seven in absentia homolog 1, Seven in absentia homolog 1 (Drosophila), Siah 1, Siah 1a, Siah E3 ubiquitin protein ligase 1, Siah-1, Siah-1a, SIAH1A, SIAH1_HUMAN, Ubiquitin ligase SIAH1

Contrôles isotype

Anticorps secondaires appropriés

Avertissement

Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.

Images (3)

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Figure 1: Western Blot - Anti-SIAH1 Antibody [ARC59737] (A309470)

Western blot analysis of various lysates, using Anti-SIAH1 Antibody [ARC59737] (A309470) at 1:2,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

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Figure 2: Western Blot - Anti-SIAH1 Antibody [ARC59737] (A309470)

Western blot analysis of Mouse liver, using Anti-SIAH1 Antibody [ARC59737] (A309470) at 1:2,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

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Figure 3: Immunohistochemistry - Anti-SIAH1 Antibody [ARC59737] (A309470)

Immunohistochemistry analysis of paraffin-embedded human kidney using Anti-SIAH1 Antibody [ARC59737] (A309470) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.