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Anti-SKIP Anticorps (A15908)

Nom du produit
Anti-SKIP Antibody
Description du produit
Rabbit polyclonal antibody to SKIP.
Applications
WB, ICC/IF
Recommander des dilutions
WB: 1:500-1:1,000, ICC/IF: 1:50-1:200
Reactivité
Human, Mouse, Rat
Immunogène
Recombinant fusion protein containing a sequence corresponding to amino acids 149-448 of human INPP5K (NP_057616.2).
Séquence
HLPPHISNNYQRLEHFDRILEMQNCEGRDIPNILDHDLIIWFGDMNFRIEDFGLHFVRESIKNRCYGGLWEKDQLSIAKKHDPLLREFQEGRLLFPPTYKFDRNSNDYDTSEKKRKPAWTDRILWRLKRQPCAGPDTPIPPASHFSLSLRGYSSHMTYGISDHKPVSGTFDLELKPLVSAPLIVLMPEDLWTVENDMMVSYSSTSDFPSSPWDWIGLYKVGLRDVNDYVSYAWVGDSKVSCSDNLNQVYIDISNIPTTEDEFLLCYYSNSLRSVVGISRPFQIPPGSLREDPLGEAQPQI
Hôte
Rabbit
Clonalité
Polyclonal
Isotype
IgG
Conjuguer

Unconjugated

Purification
Affinity purification.
Masse moléculaire
51 kDa
Forme du produit
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.
Stockage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonymes
43 kDa form skeletal muscle and kidney enriched inositol phosphatase, Inositol polyphosphate 5-phosphatase K, INP5K_HUMAN, INPP5K, muscle and kidney-enriched inositol phosphatase, PPS, skeletal muscle and kidney enriched inositol phosphatase, Skeletal muscle and kidney-enriched inositol phosphatase
Contrôles isotype
Anticorps secondaires appropriés
Avertissement
Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.
Western Blot - Anti-SKIP Antibody (A15908) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 1: Western Blot - Anti-SKIP Antibody (A15908)
Western blot analysis of extracts of 293T cells, using Anti-SKIP Antibody (A15908) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.
Western Blot - Anti-SKIP Antibody (A15908) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 2: Western Blot - Anti-SKIP Antibody (A15908)
Western blot analysis of extracts of Mouse skeletal muscle, using Anti-SKIP Antibody (A15908) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 60s.
Immunofluorescence - Anti-SKIP Antibody (A15908) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 3: Immunofluorescence - Anti-SKIP Antibody (A15908)
Immunofluorescence analysis of A-549 cells using Anti-SKIP Antibody (A15908) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
Immunofluorescence - Anti-SKIP Antibody (A15908) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 4: Immunofluorescence - Anti-SKIP Antibody (A15908)
Immunofluorescence analysis of HeLa cells using Anti-SKIP Antibody (A15908) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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