Anti-Smad2 Anticorps [ARC0343] (A307856)

Name: Anti-Smad2 Antibody [ARC0343]
Brand: Antibodies.com
SKU: A307856
Price: 470.0 USD
Availability: InStock

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$470

Anticorps monoclonal [ARC0343] de lapin contre Smad2 pour WB, ICC/IF et IP.

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Garantie de Correspondance des Prix

Livraison

Frais de livraison

$40

Date de livraison

Livraison sous 5-8 jours ouvrables.

Nous ContacterContact

Téléphone

+1 (314) 370-6046

E-mail

orders@antibodies.com

Garanties

Qualité 100 % Garantie

Garantie de Correspondance des Prix

Spécifications

Nom du produit

Anti-Smad2 Antibody [ARC0343]

Description du produit

Rabbit monoclonal [ARC0343] antibody to Smad2.

Applications

WB, ICC/IF, IP

Recommander des dilutions

WB: 1:500-1:2,000, ICC/IF: 1:50-1:200, IP: 1:500-1:1,000

Reactivité

Human, Mouse, Rat

Immunogène

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Smad2 (Q15796).

Séquence

MSSILPFTPPVVKRLLGWKKSAGGSGGAGGGEQNGQEEKWCEKAVKSLVKKLKKTGRLDELEKAITTQNCNTKCVTIPSTCSEIWGLSTPNTIDQWDTTG

Hôte

Rabbit

Clonalité

Monoclonal

Clone

ARC0343

Isotype

IgG

Conjuguer

Unconjugated

Purification

Affinity purification.

Masse moléculaire

58 kDa

Forme du produit

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Stockage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonymes

Drosophila, homolog of, MADR2, hMAD-2, HsMAD2, JV18, JV18-1, JV181, MAD, MAD homolog 2, MAD Related Protein 2, Mad-related protein 2, MADH2, MADR2, MGC22139, MGC34440, Mother against DPP homolog 2, Mothers against decapentaplegic homolog 2, Mothers against decapentaplegic, Drosophila, homolog of, 2, Mothers against DPP homolog 2, OTTHUMP00000163489, Sma and Mad related protein 2, Sma- and Mad-related protein 2 MAD, SMAD 2, SMAD family member 2, SMAD, mothers against DPP homolog 2, SMAD2_HUMAN

Contrôles isotype

Anticorps secondaires appropriés

Avertissement

Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.

Images (5)

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Figure 1: Western Blot - Anti-Smad2 Antibody [ARC0343] (A307856)

Western blot analysis of extracts from wild type (WT) and Smad2 knockdown (KD) HeLa cells, using Anti-Smad2 Antibody [ARC0343] (A307856) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.

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Figure 2: Western Blot - Anti-Smad2 Antibody [ARC0343] (A307856)

Western blot analysis of extracts of various cell lines, using Anti-Smad2 Antibody [ARC0343] (A307856) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.

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Figure 3: Western Blot - Anti-Smad2 Antibody [ARC0343] (A307856)

Western blot analysis of extracts of Rat lung, using Anti-Smad2 Antibody [ARC0343] (A307856) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 3min.

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Figure 4: Immunofluorescence - Anti-Smad2 Antibody [ARC0343] (A307856)

Immunofluorescence analysis of NIH-3T3 cells using Anti-Smad2 Antibody [ARC0343] (A307856) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).