Anti-Smad4 Anticorps [ARC5009-06] (A308139)

Name: Anti-Smad4 Antibody [ARC5009-06]
Brand: Antibodies.com
SKU: A308139
Price: 470.0 USD
Availability: InStock

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$470

Anticorps monoclonal [ARC5009-06] de lapin contre Smad4 pour WB, IHC, IP et ChIP.

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Garantie de Correspondance des Prix

Livraison

Frais de livraison

$40

Date de livraison

Livraison sous 5-8 jours ouvrables.

Nous ContacterContact

Téléphone

+1 (314) 370-6046

E-mail

orders@antibodies.com

Garanties

Qualité 100 % Garantie

Garantie de Correspondance des Prix

Mots clés :

Anticorps Validé par Knockout

Spécifications

Nom du produit

Anti-Smad4 Antibody [ARC5009-06]

Description du produit

Rabbit monoclonal [ARC5009-06] antibody to Smad4.

Applications

WB, IHC, IP, ChIP

Recommander des dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200, IP: 1:500-1:1,000, ChIP: 1:500-1:1,000, CUT&Tag: 1⁶ cells/1µg

Reactivité

Human, Mouse, Rat

Immunogène

Recombinant fusion protein containing a sequence corresponding to amino acids 320-552 of human Smad4 (NP_005350.1).

Séquence

PEYWCSIAYFEMDVQVGETFKVPSSCPIVTVDGYVDPSGGDRFCLGQLSNVHRTEAIERARLHIGKGVQLECKGEGDVWVRCLSDHAVFVQSYYLDREAGRAPGDAVHKIYPSAYIKVFDLRQCHRQMQQQAATAQAAAAAQAAAVAGNIPGPGSVGGIAPAISLSAAAGIGVDDLRRLCILRMSFVKGWGPDYPRQSIKETPCWIEIHLHRALQLLDEVLHTMPIADPQPLD

Hôte

Rabbit

Clonalité

Monoclonal

Clone

ARC5009-06

Isotype

IgG

Conjuguer

Unconjugated

Purification

Affinity purification.

Masse moléculaire

65 kDa

Forme du produit

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.01% Thiomersal.

Stockage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonymes

(Small) Mothers Against Decapentaplegic, Deleted in Pancreatic Carcinoma, Deleted in Pancreatic Carcinoma 4, Deleted in pancreatic carcinoma locus 4, Deletion target in pancreatic carcinoma 4, DPC 4, DPC4, hSMAD4, JIP, MAD homolog 4, MAD mothers against decapentaplegic Drosophila homolog 4, MAD mothers against decapentaplegic homolog 4, MADH 4, MADH4, Med, Medea, Mothers against decapentaplegic homolog 4, Mothers against decapentaplegic, Drosophila, homolog of, 4, Mothers against DPP homolog 4, MYHRS, OTTHUMP00000163548, SMA- and MAD-related protein 4, SMAD 4, SMAD family member 4, SMAD mothers against DPP homolog 4, SMAD4_HUMAN

Contrôles isotype

Anticorps secondaires appropriés

Avertissement

Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.

Images (6)

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Figure 1: Anti-Smad4 Antibody [ARC5009-06] (A308139)

CUT&Tag was performed using the CUT&Tag Assay Kit (pAG-Tn5) for Illumina from 10⁵ K562 cells with 1µg of Anti-Smad4 Antibody [ARC5009-06] (A308139), and Goat Anti-Rabbit IgG H&L Antibody. The CUT&Tag results indicate the enrichment pattern of Smad4 in representative gene loci (JUNB), as shown in figure.

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Figure 2: Western Blot - Anti-Smad4 Antibody [ARC5009-06] (A308139)

Western blot analysis of extracts of various cell lines, using Anti-Smad4 Antibody [ARC5009-06] (A308139) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.

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Figure 3: Western Blot - Anti-Smad4 Antibody [ARC5009-06] (A308139)

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Figure 4: Immunohistochemistry - Anti-Smad4 Antibody [ARC5009-06] (A308139)

Immunohistochemistry analysis of paraffin-embedded human liver cancer using Anti-Smad4 Antibody [ARC5009-06] (A308139) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.