Anti-SMC1A (phospho Ser957) Anticorps (A10885)

Name: Anti-SMC1A (phospho Ser957) Antibody
Brand: Antibodies.com
SKU: A10885
Price: 435.0 USD
Availability: InStock

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$435

Anticorps polyclonal de lapin contre SMC1A (phospho Ser957) pour WB, IHC et IP.

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Garantie de Correspondance des Prix

Livraison

Frais de livraison

$40

Date de livraison

Livraison sous 5-8 jours ouvrables.

Nous ContacterContact

Téléphone

+1 (314) 370-6046

E-mail

orders@antibodies.com

Garanties

Qualité 100 % Garantie

Garantie de Correspondance des Prix

Spécifications

Nom du produit

Anti-SMC1A (phospho Ser957) Antibody

Description du produit

Rabbit polyclonal antibody to SMC1A (phospho Ser957).

Applications

WB, IHC, IP

Recommander des dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200, IP: 1:500-1:1,000

Reactivité

Human, Mouse, Rat

Immunogène

A synthetic phosphorylated peptide around S957 of human SMC1A (NP_006297.2).

Séquence

GSSQG

Hôte

Rabbit

Clonalité

Polyclonal

Isotype

IgG

Conjuguer

Unconjugated

Purification

Affinity purification.

Masse moléculaire

160 kDa

Forme du produit

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.

Stockage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonymes

Chromosome segregation protein SmcB, DXS423E, KIAA0178, MGC138332, Sb1.8, Segregation of mitotic chromosomes 1, SMC protein 1A, SMC-1-alpha, SMC-1A, SMC1, SMC1 (structural maintenance of chromosomes 1 yeast) like 1, SMC1 structural maintenance of chromosomes 1 like 1, SMC1alpha, SMC1A_HUMAN, SMC1L1, SMCB, Structural maintenance of chromosomes 1A, Structural maintenance of chromosomes protein 1A

Contrôles isotype

Anticorps secondaires appropriés

Avertissement

Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.

Images (4)

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Figure 1: Western Blot - Anti-SMC1A (phospho Ser957) Antibody (A10885)

Western blot analysis of extracts of various cell lines, using Anti-SMC1A (phospho Ser957) Antibody (A10885) at 1:1,000 dilution. HeLa and NIH/3T3 cells were treated by UV for 15-30 minutes. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% BSA. Detection was with a ECL Basic Kit. Exposure time: 3s.

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Figure 2: Western Blot - Anti-SMC1A (phospho Ser957) Antibody (A10885)

Western blot analysis of extracts of HeLa cells, using Anti-SMC1A (phospho Ser957) Antibody (A10885) at 1:1,000 dilution or SMC1A antibody (A7008). HeLa cells were treated by UV at room temperature for 15-30 minutes. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% BSA. Detection was with a ECL Basic Kit. Exposure time: 3s.

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Figure 3: Immunohistochemistry - Anti-SMC1A (phospho Ser957) Antibody (A10885)

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Anti-SMC1A (phospho Ser957) Antibody (A10885) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 4: Western Blot - Anti-SMC1A (phospho Ser957) Antibody (A10885)

Immunoprecipitation analysis of 300µg extracts of HeLa cells using 3µg of Anti-SMC1A (phospho Ser957) Antibody (A10885). This Western blot was performed on the immunoprecipitate using Anti-SMC1A (phospho Ser957) Antibody (A10885) at a dilution of 1:1000.