Anti-Sonic Hedgehog Anticorps [ARC0701] (A308161)

Name: Anti-Sonic Hedgehog Antibody [ARC0701]
Brand: Antibodies.com
SKU: A308161

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Ce produit est discontinué

Anti-Sonic Hedgehog Anticorps [ARC0701] (A308161) a été abandonné et n'est plus disponible.

Voir toutes Anti-Sonic Hedgehog Anticorps.

Spécifications

Nom du produit

Anti-Sonic Hedgehog Antibody [ARC0701]

Description du produit

Rabbit monoclonal [ARC0701] antibody to Sonic Hedgehog.

Applications

WB, ICC/IF

Recommander des dilutions

WB: 1:500-1:2,000, ICC/IF: 1:50-1:200

Reactivité

Human, Mouse, Rat

Immunogène

A synthetic peptide corresponding to a sequence within amino acids 200-300 of human Sonic Hedgehog (Shh) (Q15465).

Séquence

PGSATVHLEQGGTKLVKDLSPGDRVLAADDQGRLLYSDFLTFLDRDDGAKKVFYVIETREPRERLLLTAAHLLFVAPHNDSATGEPEASSGSGPPSGGALG

Hôte

Rabbit

Clonalité

Monoclonal

Clone

ARC0701

Isotype

IgG

Conjuguer

Unconjugated

Purification

Affinity purification.

Masse moléculaire

50 kDa / 27 kDa

Forme du produit

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Stockage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonymes

HHG 1, HHG-1, HHG1, HLP 3, HLP3, Holoprosencephaly 3, HPE 3, HPE3, MCOPCB5, shh, SHH_HUMAN, SMMC I, SMMCI, Sonic Hedgehog (Drosophila) homolog, Sonic hedgehog homolog, sonic hedgehog homolog (Drosophila), Sonic hedgehog protein, Sonic hedgehog protein C-product, TPT, TPTPS

Contrôles isotype

Anticorps secondaires appropriés

Avertissement

Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.

Images (3)

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Figure 1: Western Blot - Anti-Sonic Hedgehog Antibody [ARC0701] (A308161)

Western blot analysis of extracts of various cell lines, using Anti-Sonic Hedgehog Antibody [ARC0701] (A308161) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 60s.

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Figure 2: Western Blot - Anti-Sonic Hedgehog Antibody [ARC0701] (A308161)

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Figure 3: Immunofluorescence - Anti-Sonic Hedgehog Antibody [ARC0701] (A308161)

Immunofluorescence analysis of Hep G2 cells using Anti-Sonic Hedgehog Antibody [ARC0701] (A308161) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).