Anti-Synaptotagmin VII Anticorps [S275-14] (A304818)

Name: Anti-Synaptotagmin VII Antibody [S275-14]
Brand: Antibodies.com
SKU: A304818
Price: 500.0 USD
Availability: InStock

Cliquez pour ouvrir le point de vue élargi

$500

Anticorps monoclonal [S275-14] de souris contre Synaptotagmin VII pour WB, IHC et ICC/IF.

Qualité 100 % Garantie

Garantie de Correspondance des Prix

Livraison

Frais de livraison

$40

Date de livraison

Livraison sous 5-8 jours ouvrables.

Nous ContacterContact

Téléphone

+1 (314) 370-6046

E-mail

orders@antibodies.com

Garanties

Qualité 100 % Garantie

Garantie de Correspondance des Prix

Spécifications

Nom du produit

Anti-Synaptotagmin VII Antibody [S275-14]

Description du produit

Mouse monoclonal [S275-14] antibody to Synaptotagmin VII.

Spécificité

This antibody can identify other isoforms bands at ~65kD.

Applications

WB, IHC, ICC/IF

Recommander des dilutions

WB: 1:1,000, ICC/IF: 1:100

Reactivité

Human, Mouse, Rat

Réactivité croisée

This antibody does not cross-react with Synaptotagmin 6.

Immunogène

Fusion protein amino acids 150-239 (Cytoplasmic C2A domain) of mouse Synaptotagmin-7.

Hôte

Mouse

Clonalité

Monoclonal

Clone

S275-14

Isotype

IgG2b

Conjuguer

Unconjugated

Purification

Protein G purification.

Concentration

1 mg/ml

Masse moléculaire

~45 kDa

Forme du produit

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.4, with 50% Glycerol and 0.09% Sodium Azide.

Stockage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonymes

IPCA 7, IPCA-7, IPCA7, PCANAP7, prostate cancer associated protein 7, Prostate cancer-associated protein 7, Synaptotagmin-7, Synaptotagmin7, SynaptotagminVII, Synatotagmin 7, SYT 7, SYT VII, SYT7, SYT7_HUMAN, SytVII

Contrôles isotype

Anticorps secondaires appropriés

Avertissement

Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.

Images (3)

Cliquez pour ouvrir le point de vue élargi

Figure 1: Immunofluorescence - Anti-Synaptotagmin VII Antibody [S275-14] (A304818)

Immunocytochemistry/Immunofluorescence analysis of human neuroblastoma cells (SH-SY5Y), fixed in 4% PFA for 15 min, using Anti-Synaptotagmin VII Antibody [S275-14] (A304818), at 1:100 for overnight at 4°C with slow rocking. The secondary antibody used was AlexaFluor 488 at 1:1,000 for 1 hour at room temperature. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 minutes at room temperature.(A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) Synaptotagmin-7 Antibody (D) Composite.

Cliquez pour ouvrir le point de vue élargi

Figure 2: Western Blot - Anti-Synaptotagmin VII Antibody [S275-14] (A304818)

Western blot analysis of rat brain lysates showing detection of Synaptotagmin 7 protein using Anti-Synaptotagmin VII Antibody [S275-14] (A304818) at 1:100, 1:250, 1:500, and 1:1,000.

Cliquez pour ouvrir le point de vue élargi

Figure 3: Immunofluorescence - Anti-Synaptotagmin VII Antibody [S275-14] (A304818)

Immunocytochemistry/Immunofluorescence analysis of human neuroblastoma cell line (SK-N-BE, fixed in 4% formaldehyde for 15 min at room temperature, using Anti-Synaptotagmin VII Antibody [S275-14] (A304818), at 1:100 for 60 minutes at room temperature. The secondary antibody used was Goat Anti-Mouse ATTO 488 at 1:100 for 60 minutes at room temperature. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000; 1:5,000 for 60 minutes room temperature, 5 minutes room temperature. Localization: Cytoplasmic Vesicle, Nucleus. Magnification: 60X.(A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) Synaptotagmin-7 Antibody. (D) Composite.