Synthetic nanodiscs offer a stable and biologically relevant environment that closely mimics cell membranes and enables full-length transmembrane human GPCR C5L2 protein to be purified and analysed in vitro.
Applications
ELISA, SDS-PAGE
Système d'expression
HEK293 cells
Nature
Synthetic
Espèces de protéines
Human
Longueur des protéines
Full length protein.
Masse moléculaire
Full length human GPCR C5L2 protein has a MW of 36.1 kDa.
Conjuguer
Unconjugated
Forme du produit
Lyophilized
Concentration
Reconstitution dependent.
Formulation
Lyophilized from nanodisc solubilization buffer (20mM Tris-HCl, 150mM NaCl, pH 8.0). Normally 5%–8% Trehalose is added as a protectant before lyophilization.
Stockage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C. Reconstituted: Aliquot and store at -80°C. Product is stable for one year. Avoid freeze/thaw cycles.
Synonymes
C5a anaphylatoxin chemotactic receptor C5L2, C5ARL_HUMAN, C5L2, E030029A11Rik, G protein coupled receptor 77, G protein coupled receptor C5L2, G-protein coupled receptor 77, GPF77, GPR77
Avertissement
Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.
Figure 1: Synthetic Nanodisc Human GPCR C5L2 Protein (A318438)
ELISA plates were pre-coated with Synthetic Nanodisc Human GPCR C5L2 Protein (A318438) (0.2 µg/well). Serial diluted Anti-Flag Tag Antibody solutions were added, washed, and incubated with secondary antibody before ELISA reading. From this data, the EC50 for Anti-Flag Tag Antibody binding with GPR77-Nanodisc is 3.415 µg/ml.
Figure 3: Synthetic Nanodisc Human GPCR C5L2 Protein (A318438)
Diagram showing how synthetic nanodiscs containing full-length multi-pass transmembrane proteins in a phospholipid bilayer are generated from native cell membranes.
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