Synthetic nanodiscs offer a stable and biologically relevant environment that closely mimics cell membranes and enables full-length transmembrane human GPCR GPR75 protein to be purified and analysed in vitro.
Applications
Western Blot, ELISA
Système d'expression
HEK293 cells
Nature
Synthetic
Espèces de protéines
Human
Longueur des protéines
Full length protein.
Masse moléculaire
Full length human GPCR GPR75 protein has a MW of 59.4 kDa.
Conjuguer
Unconjugated
Forme du produit
Lyophilized
Concentration
Reconstitution dependent.
Formulation
Lyophilized from nanodisc solubilization buffer (20mM Tris-HCl, 150mM NaCl, pH 8.0). Normally 5%–8% Trehalose is added as a protectant before lyophilization.
Stockage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C. Reconstituted: Aliquot and store at -80°C. Product is stable for one year. Avoid freeze/thaw cycles.
Synonymes
G protein coupled receptor 75, GPR chr2, Gpr75, GPR75_HUMAN, GPRchr2, OTTHUMP00000159608, Probable G protein coupled receptor 75, Probable G-protein coupled receptor 75, WI 31133, WI31133
Avertissement
Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.
Figure 1: Synthetic Nanodisc Human GPCR GPR75 Protein (A318467)
ELISA plates were pre-coated with Synthetic Nanodisc Human GPCR GPR75 Protein (A318467) (0.2 µg/well). Serial diluted Anti-Flag Tag Antibody solutions were added, washed, and incubated with secondary antibody before ELISA reading. From this data, the EC50 for Anti-Flag Tag Antibody binding with GPR75-Nanodisc is 3.747 µg/ml.
Figure 2: Western Blot - Synthetic Nanodisc Human GPCR GPR75 Protein (A318467)
Western blot analysis of Synthetic Nanodisc Human GPCR GPR75 Protein (A318467) with Anti-Flag Tag Antibody at 1:5,000 dilution, followed by Goat Anti-Rabbit IgG Antibody (HRP) at 1:5,000 dilution.
Figure 3: Synthetic Nanodisc Human GPCR GPR75 Protein (A318467)
Diagram showing how synthetic nanodiscs containing full-length multi-pass transmembrane proteins in a phospholipid bilayer are generated from native cell membranes.
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