Synthetic nanodiscs offer a stable and biologically relevant environment that closely mimics cell membranes and enables full-length transmembrane human GPCR GPRC5D protein to be purified and analysed in vitro.
Applications
ELISA, SDS-PAGE
Système d'expression
HEK293 cells
Nature
Synthetic
Espèces de protéines
Human
Longueur des protéines
Full length protein.
Masse moléculaire
Full length human GPCR GPRC5D protein has a MW of 38.6 kDa.
Conjuguer
Unconjugated
Forme du produit
Lyophilized
Concentration
Reconstitution dependent.
Formulation
Lyophilized from nanodisc solubilization buffer (20mM Tris-HCl, 150mM NaCl, pH 8.0). Normally 5%–8% Trehalose is added as a protectant before lyophilization.
Stockage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C. Reconstituted: Aliquot and store at -80°C. Product is stable for one year. Avoid freeze/thaw cycles.
Synonymes
G protein coupled receptor family C group 5 member D, G-protein coupled receptor family C group 5 member D, GPC5D_HUMAN, GPRC5D
Avertissement
Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.
Figure 1: Synthetic Nanodisc Human GPCR GPRC5D Protein (A318486)
ELISA plates were added with Flag Tag Synthetic Nanodisc Human GPRC5D Protein (A318486) (0.5 µg/per well) on an Anti-Flag Tag Antibody pre-coated (0.5 µg/per well) plate. Serial diluted Anti-GPRC5D Antibody [DM90] (Biotin) - Azide free (A318615) solutions were added, washed, and incubated with secondary antibody before ELISA reading. From above data, the EC50 for Anti-GPRC5D Antibody [DM90] (Biotin) - Azide free (A318615) binding with Synthetic Nanodisc Human GPRC5D Protein (A318486) is 32.86 µg/ml.
Figure 3: Synthetic Nanodisc Human GPCR GPRC5D Protein (A318486)
Diagram showing how synthetic nanodiscs containing full-length multi-pass transmembrane proteins in a phospholipid bilayer are generated from native cell membranes.
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