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TAF1A Cell Based Kit Elisa (A103587)

Nom du produit
TAF1A Cell Based ELISA Kit
Type de test
Cell-based (qualitative)
Principe du test
This TAF1A Cell Based ELISA Kit allows for the detection of TAF1A and the effects that certain stimulation conditions have on TAF1A expression in different cell lines. Qualitative determination of TAF1A concentration is achieved by an indirect ELISA format. In essence, the TAF1A is captured by Anti-TAF1A Antibodies which in turn are detected by HRP-conjugated secondary antibodies. Through this binding, the HRP enzyme (conjugated to the secondary antibody) can catalyze a colorimetric reaction upon substrate addition. Due to the qualitative nature of this TAF1A Cell Based ELISA Kit, multiple normalization methods are described: 1) Anti-GAPDH Antibody is included to serve as an internal positive control in normalizing the target absorbance values. 2) Following the colorimetric measurement of HRP activity via substrate addition, the Crystal Violet whole-cell staining method is used to determine cell density. After staining, the results can be analyzed by normalizing the absorbance values to cell amounts, by which the plating difference can be adjusted.
Plate-forme
Microplate
Méthode de détection
Colorimetric
Type d'échantillon
Adherent cells and suspension cells.
Gamme de detection
> 5000 Cells
Durée du test
4 hours 30 minutes
Reactivité
Human, Rat
Precision
Intra-assay CV: < 8%, Inter-assay CV: < 10%
Stockage
Store at + 4°C. Stable for 6 months.
Synonymes
MGC:17061, RAFI48, RNA polymerase I-specific TBP-associated factor 48 kDa, SL1, SL1 48kD subunit, TAF1 48, TAF1A_HUMAN, TAFI48, TATA box binding protein (TBP) associated factor RNA polymerase I, A 48kDa, TATA box binding protein (Tbp)-associated factor, RNA polymerase I, A, TATA box binding protein associated factor 1A, TATA box binding protein associated factor RNA polymerase I subunit A, TATA box-binding protein-associated factor 1A, TATA box-binding protein-associated factor RNA polymerase I subunit A, TBP associated factor RNA polymerase I 48 kDa, TBP-associated factor 1A, Transcription factor SL1, Transcription initiation factor SL1/TIF-IB subunit A
Avertissement
Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.
Western Blot - TAF1A Cell Based ELISA Kit (CB6023) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 1: Western Blot - TAF1A Cell Based ELISA Kit (CB6023)
Western blot analysis of extracts from HeLa cells, using Anti-TAF1A Antibody. The lane on the right is treated with the synthesized peptide.
Protocol Diagram - TAF1A Cell Based ELISA Kit (CB6023) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 2: TAF1A Cell Based ELISA Kit (CB6023)
A diagram showing the protocol for TAF1A Cell Based ELISA Kit.
Protocol Summary - TAF1A Cell Based ELISA Kit (CB6023) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 3: TAF1A Cell Based ELISA Kit (CB6023)
A summary of the protocol for TAF1A Cell Based ELISA Kit.
Western Blot - Control Antibody - TAF1A Cell Based ELISA Kit (CB6023) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 4: Western Blot - TAF1A Cell Based ELISA Kit (CB6023)
The mouse monoclonal antibody to GAPDH is used as a positive control inTAF1A Cell Based ELISA Kit. Anti-GAPDH Antibody was tested for specificity by western blot with tissue lysates from human, mouse, and rat.
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