Anti-Tissue Factor Anticorps [ARC60559] (A309523)

Western blot analysis of various lysates, using Anti-Tissue Factor Antibody [ARC60559] (A309523) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.

Immunohistochemistry analysis of paraffin-embedded human lung cancer using Anti-Tissue Factor Antibody [ARC60559] (A309523) at a dilution of 1:400 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Immunofluorescence analysis of A-431 and MCF7(Negative sample) cells using Anti-Tissue Factor Antibody [ARC60559] (A309523) at a dilution of 1:300 (40x lens). DAPI was used to stain the cell nuclei (blue).