Anti-TopBP1 Anticorps (A295269)

Name: Anti-TopBP1 Antibody
Brand: Antibodies.com
SKU: A295269
Price: 505.0 USD
Availability: InStock

Ce produit est identique à Abcam (ab2402). Découvrez plus pour moins cher.

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$505

Anticorps polyclonal de lapin contre TopBP1 pour WB et IP.

Qualité 100 % Garantie

Garantie de Correspondance des Prix

Livraison

Frais de livraison

$40

Date de livraison

Livraison sous 5-8 jours ouvrables.

Nous ContacterContact

Téléphone

+1 (314) 370-6046

E-mail

orders@antibodies.com

Garanties

Qualité 100 % Garantie

Garantie de Correspondance des Prix

Spécifications

Nom du produit

Anti-TopBP1 Antibody

Description du produit

Rabbit polyclonal antibody to TopBP1.

Applications

WB, IP

Recommander des dilutions

IP: 2-5 µg / 1 mg lysate, WB: 1:2,000-1:10,000

Reactivité

Human, Mouse

Immunogène

Synthetic peptide within amino acids 1400 and the C-terminus of human TopBP1 (NP_008958. 1).

Hôte

Rabbit

Clonalité

Polyclonal

Isotype

IgG

Conjuguer

Unconjugated

Purification

Antigen affinity purification.

Concentration

1 mg/ml

Forme du produit

Liquid

Formulation

Supplied in Tris-Citrate/Phosphate Buffer, pH 7-8, with 0.09% Sodium Azide.

Stockage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Notes générales

This antibody was affinity purified using the immunising peptide immobilized on solid support. Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1. 4 equals 1.0 mg of IgG.

Synonymes

DNA topoisomerase 2 binding protein 1, DNA topoisomerase 2-binding protein 1, DNA topoisomerase II binding protein, DNA topoisomerase II binding protein 1, DNA topoisomerase II-beta-binding protein 1, DNA topoisomerase II-binding protein 1, DNA topoisomerase IIbeta binding protein 1, Hypothetical protein KIAA0259 [Fragment], KIAA0259, TOP2BP1, TOPB1_HUMAN, TOPBP 1, Topoisomerase (DNA) II binding protein 1, Topoisomerase II binding protein 1

Contrôles isotype

Anticorps secondaires appropriés

Avertissement

Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.

Images (3)

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Figure 1: Immunoprecipitation - Anti-TopBP1 Antibody (A295269)

Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Anti-TopBP1 Antibody (A295269) was used for IP at 3 µg per reaction. TopBP1 was also immunoprecipitated using a previous lot of this antibody. For blotting immunoprecipitated TopBP1, Anti-TopBP1 Antibody (A295269) was used at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

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Figure 2: Western Blot - Anti-TopBP1 Antibody (A295269)

Samples: Whole cell lysate (50 µg) from TCMK-1 and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Anti-TopBP1 Antibody (A295269) was used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

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Figure 3: Western Blot - Anti-TopBP1 Antibody (A295269)

Samples: Whole cell lysate (15 µg) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Anti-TopBP1 Antibody (A295269) was used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.