Anti-Transferrin Receptor Anticorps (A308475)

Western blot analysis of HeLa, using Anti-Transferrin Receptor Antibody (A308475) at 1:900 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.

Western blot analysis of Rat spleen, using Anti-Transferrin Receptor Antibody (A308475) at 1:900 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 20s.

Immunofluorescence analysis of L929 using Anti-Transferrin Receptor Antibody (A308475) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

Immunoprecipitation analysis of 200µg extracts of Jurkat cells using 3µg of Anti-Transferrin Receptor Antibody (A308475). This Western blot was performed on the immunoprecipitate using Anti-Transferrin Receptor Antibody (A308475) at a dilution of 1:1000.