Anti-V5 Tag Anticorps (A121614)

Name: Anti-V5 Tag Antibody
Brand: Antibodies.com
SKU: A121614
Price: 535.0 USD
Availability: InStock

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$535

Anticorps polyclonal de chèvre contre V5 Tag pour WB.

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Livraison

Frais de livraison

$40

Date de livraison

Livraison sous 5-8 jours ouvrables.

Nous ContacterContact

Téléphone

+1 (314) 370-6046

E-mail

orders@antibodies.com

Garanties

Qualité 100 % Garantie

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Spécifications

Nom du produit

Anti-V5 Tag Antibody

Description du produit

Goat polyclonal antibody to V5 Tag.

Spécificité

The antibody recognizes the V5 tag fused to either the amino- or carboxy-terminal of recombinant proteins.

Applications

Recommander des dilutions

WB: 1:500-1:5,000, IF: 1:500-1:2,000

Immunogène

Recombinant peptide corresponding to amino acids 95-108 of the P and V proteins of the Paramyxovirus of simian virus SV5, expressed in and purified from E. coli.

Séquence

GKPIPNPLLGLDST

Hôte

Goat

Clonalité

Polyclonal

Isotype

IgG

Conjuguer

Unconjugated

Purification

Affinity purification.

Concentration

3 mg/ml

Forme du produit

Liquid

Formulation

Supplied in Phosphate Buffered Saline with 20% Glycerol and 0.05% Sodium Azide.

Stockage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonymes

GKPIPNPLLGLDST epitope tag, GKPIPNPLLGLDST tag, Protein Rev, Regulator of expression of viral proteins, rev, V5 epitope tag

Contrôles isotype

Goat IgG (A121671)

Anticorps secondaires appropriés

Avertissement

Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.

Images (2)

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Figure 1: Western Blot - Anti-V5 Tag Antibody (A121614)

MBP-V5 recombinant protein and 293 cells, transfected with V5-mCherry, detected with Anti-V5 Tag Antibody at a 1:1,000 dilution. Lysates at 100µg per lane and rabbit anti-goat IgG antibody (HRP) at a 1:10,000 dilution.

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Figure 2: Anti-V5 Tag Antibody (A121614)

Myo5a deficient melanocytes transiently transduced with adenovirus expressing V5-tagged Myo5a, fixed, and stained with Anti-V5 Tag Antibody at a 1:1,000 dilution. Donkey anti-goat IgG antibody (Alexa Fluor 568) was used as the secondary antibody.

Références (1)

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The Na⁺ -coupled glucose transporter SGLT2 interacts with its accessory unit MAP17 in vitro and their expressions overlap in the renal proximal tubule.

Références : Anti-V5 Tag Antibody (A121614)

Résumé :

Na⁺ -glucose cotransporter 2 is the renal Na⁺ -coupled glucose transporter responsible for the tubular glucose reabsorption, while MAP17 was recently identified as its accessory unit. Mutations in either of the proteins' coding genes, SLC5A2 and PDZK1IP1, lead to urinary glucose excretion. To investigate whether MAP17 interacts with SGLT2 in vitro, we engineered a V5-tagged SGLT2 construct and evaluated HEK293T cells coexpressing it together with a HA tagged MAP17 construct. MAP17 is shown to colocalize and coimmunoprecipitate with SGLT2. Also, in human kidney sections, the expression of both proteins overlaps at the apical surface of tubular epithelia. This interaction provides the rationale behind SGLT2 activation by MAP17 as well the similarity of the SLC5A2 and PDZK1IP1 glucosuric phenotypes.

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