Anti-GFP Antibody [ARC50809] (A307789)

Name: Anti-GFP Antibody [ARC50809]
Brand: Antibodies.com
SKU: A307789

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This product is discontinued

Anti-GFP Antibody [ARC50809] (A307789) has been discontinued and is no longer available.

View all Anti-GFP Antibodies.

Highlighted products for Anti-GFP Antibody

(7)

A290

Anti-GFP Antibody

Rabbit polyclonal antibody to GFP for WB, ELISA, ICC/IF, Flow Cytometry, IHC-Fr, IHC-P, IP and Electron Microscopy.

(7)

A290

Anti-GFP Antibody

Rabbit polyclonal antibody to GFP for WB, ELISA, ICC/IF, Flow Cytometry, IHC-Fr, IHC-P, IP and Electron Microscopy.

Specifications

Name

Anti-GFP Antibody [ARC50809]

Description

Rabbit monoclonal [ARC50809] antibody to GFP.

Applications

WB, ICC/IF

Dilutions

WB: 1:10,000-1:40,000, ICC/IF: 1:50-1:200, IP: 1:50-1:200

Reactivity

Species Independent

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-238 of GFP.

Sequence

MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKANFKTRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITHGMDELYK

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC50809

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

25 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

Green fluorescent protein

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (2)

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Figure 1: Western Blot - Anti-GFP Antibody [ARC50809] (A307789)

Western blot analysis of extracts of normal Eukaryotic expression of GFP and Eukaryotic expression of GFP transfected with GFP Protein, using Anti-GFP Antibody [ARC50809] (A307789) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.

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Figure 2: Immunofluorescence - Anti-GFP Antibody [ARC50809] (A307789)

Immunofluorescence analysis of 293T and 293T-GFP using Anti-GFP Antibody [ARC50809] (A307789) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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