Anti-GFP Antibody [3B11] (A85303)

Mouse monoclonal (3B11) antibody to GFP for WB, ICC/IF and IHC.

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4-6 Business Days
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+1 (314) 370-6046
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Email
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Highlighted products for Anti-GFP Antibody

Name
Anti-GFP Antibody [3B11]
Description
Mouse monoclonal (3B11) antibody to GFP.
Specificity
The epitope for this antibody is in the peptide MVSKGAELFTGIVPILIE, the N-terminal 18 amino acids of the protein, which is found in most commercial GFP vectors and is distinct from the sequence seen in other fluorescent proteins. The homologous region of eGFP is MVSKGEELFTGVVPILVE, and this antibody binds this peptide also.
Applications
WB, ICC/IF, IHC
Dilutions
WB: 1:1,000-1:5,000, ICC/IF: 1:1,000-1:5,000, IHC: 1:1,000-1:5,000
Reactivity
AcGFP, eGFP
Cross Reactivity
This antibody does not recognize mCherry.
Immunogen
Recombinant Aequoria coerulescens GFP protein, expressed in and purified from E. coli.
Sequence
MVSKGAELFTGIVPILIELNGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLSYGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFEDDGNYKSRAEVKFEGDTLVNRIELTGTDFKEDGNILGNKMEYNYNAHNVYIMTDKAKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMIYFGFVTAAAITHGMDELYK
Host
Mouse
Clonality
Monoclonal
Clone ID
3B11
Isotype
IgM
Conjugate

Unconjugated

Purification
Immunogen affinity purification.
Concentration
1 mg/ml
Molecular Weight
27 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 50% Glycerol and 5mM Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
General Notes
This antibody can be used to verify the expression, size, and stability of both AcGFP and eGFP fusion proteins in western blotting experiments, and to amplify GFP signals in tissues of transgenic animals.
Synonyms
Green fluorescent protein
Isotype Controls
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
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