Anti-GSK3 beta Antibody (A305082)

Name: Anti-GSK3 beta Antibody
Brand: Antibodies.com
SKU: A305082
Price: 425.0 USD
Availability: InStock

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$425

Rabbit polyclonal antibody to GSK3 beta for WB, IHC and ICC/IF.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Telephone

+1 (314) 370-6046

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Specifications

Name

Anti-GSK3 beta Antibody

Description

Rabbit polyclonal antibody to GSK3 beta.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:2,000, ICC/IF: 1:200

Reactivity

Human, Mouse, Rat, Bovine

Immunogen

Synthetic peptide corresponding to the sequence near the C-terminus of human GSK-3Beta.

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Protein A purification.

Concentration

1.3 mg/ml

Molecular Weight

~47 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.4.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

Glycogen Synthase Kinase 3 Beta, Glycogen synthase kinase-3 beta, GSK 3 beta, GSK-3 beta, GSK3B, GSK3beta isoform, GSK3B_HUMAN, Serine/threonine-protein kinase GSK3B

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (3)

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Figure 1: Immunofluorescence - Anti-GSK3 beta Antibody (A305082)

Immunocytochemistry/Immunofluorescence analysis of human heat shocked cervical cancer cell line (HeLa), fixed in 2% formaldehyde for 20 minutes at room temperature, using Anti-GSK3 beta Antibody (A305082), at 1:100 for 12 hours at 4°C. The secondary antibody used was R-PE Goat Anti-Rabbit (yellow) at 1:200 for 2 hours at room temperature. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at room temperature. Localization: Cytoplasm. Nucleus. Magnification: 20x.(A) DAPI (blue) nuclear stain. (B) Anti-GSK3beta Antibody. (C) Composite. Heat Shocked at 42°C for 1h.

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Figure 2: Western Blot - Anti-GSK3 beta Antibody (A305082)

Western blot analysis of human Cell line lysates showing detection of GSK3 Beta protein using Anti-GSK3 beta Antibody (A305082) at 1:1,000 for 2 hours at room temperature. Load: 15 µgprotein. Block: 1.5% BSA for 30 minutes at room temperature. The secondary antibody used was Donkey Anti-Rabbit IgG: HRP for 1 hour at room temperature.

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Figure 3: Immunofluorescence - Anti-GSK3 beta Antibody (A305082)

Immunocytochemistry/Immunofluorescence analysis of human heat shocked cervical cancer cell line (HeLa), fixed in 2% formaldehyde for 20 minutes at room temperature, using Anti-GSK3 beta Antibody (A305082), at 1:100 for 12 hours at 4°C. The secondary antibody used was APC Goat Anti-Rabbit (red) at 1:200 for 2 hours at room temperature. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at room temperature. Localization: Cytoplasm. Nucleus. Magnification: 100x.(A) DAPI (blue) nuclear stain. (B) Anti-GSK3beta Antibody. (C) Composite. Heat Shocked at 42°C for 1h.

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